A spectrophotometric study on the interaction of neutral red with double-stranded DNA in large excess

被引：95

作者：

Huang, CZ ^{[1
]}

Li, YF ^{[1
]}

Feng, P ^{[1
]}

机构：

[1] SW Normal Univ, Inst Environm Chem, Chongqing 400715, Peoples R China

来源：

TALANTA | 2001年 / 55卷 / 02期

基金：

中国国家自然科学基金;

关键词：

neutral red; DNA; interaction; spectrophotometry;

D O I：

10.1016/S0039-9140(01)00428-3

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

With the measurement of molecular absorption, the interaction of neutral red (NR) with double-stranded DNA in large excess was investigated. It was found that the interaction of NR, existing in the acidic state (HNR) at pH 4.56, with double-stranded structure DNA displays different spectral features depending on the molar ratio of HNR/DNA, R. If R > 1.33, the binding process is characterized by a binding constant at the 10(6) level with each nucleotide residue of double-stranded DNA binding one HNR molecule. If R < 0.67, the binding constant is reduced to the 10(4) level, and the binding number for each nucleotide residue of double-stranded DNA to HNR is less than one. (C) 2001 Elsevier Science B.V. All rights reserved.

引用

页码：321 / 328

页数：8

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