Influence of solvent uptake and swelling by poly(styrene-divinylbenzene) column packings on sample sorption rate and band broadening in reversed-phase liquid chromatography

被引:26
作者
Ells, B [1 ]
Wang, Y [1 ]
Cantwell, FF [1 ]
机构
[1] Univ Alberta, Dept Chem, Edmonton, AB T6G 2G2, Canada
基金
加拿大自然科学与工程研究理事会;
关键词
solvent uptake; swelling; band broadening; sorption rate; sorption isotherms; tetrahydrofuran;
D O I
10.1016/S0021-9673(98)01070-X
中图分类号
Q5 [生物化学];
学科分类号
071010 ; 081704 ;
摘要
Porous poly(styrene-divinylbenzene) (PS-DVB) HPLC packings give broad, tailed peaks for some types of solutes. This phenomenon is due to sorption of these solutes into the polymer matrix where they experience very slow, hindered diffusion. It is known that the presence of small amounts of tetrahydrofuran (THF) in the methanol (MeOH)-H2O mobile phase yields narrower and less tailed peaks for such solutes. In this study, Hamilton PRP-infinity, a nonporous PS-DVB polymer, serves as a model for the matrix of porous polymers. The following measurements were made: sorption isotherms for MeOH and THF from aqueous solution; swelling of PRP-infinity as a function of activity of MeOH and THF in aqueous solution; and both sorption isotherms and sorption rate curves for the solute naphthalene from various solvent mixtures. The addition of 2% THF to 70:30 MeOH-H2O produced an additional 0.4% swelling, an 11% decrease in the sorption capacity and a 90% increase in the diffusion coefficient of naphthalene in PRP-infinity. The increase in the diffusion coefficient, which is responsible for the improved elution peak shape of naphthalene, is shown to be caused by the decrease in sorption capacity, rather than by the additional swelling. The sorbed THF serves to fill and "block" the smallest, most highly hindered micropores in the polymer matrix. (C) 1999 Elsevier Science B.V. All rights reserved.
引用
收藏
页码:3 / 18
页数:16
相关论文
共 65 条
  • [1] POROUS POLYMERS AS AN ANCHOR FOR CATALYSIS
    ALBRIGHT, RL
    [J]. REACTIVE POLYMERS, 1986, 4 (02): : 155 - 174
  • [2] [Anonymous], 1965, ENCY POLYM SCI TECHN
  • [3] ARSHADY R, 1988, MAKROMOL CHEM, V189, P1295
  • [4] Barton AF., 2017, CRC handbook of solubility parameters and other cohesion parameters
  • [5] LIQUID-CHROMATOGRAPHY COLUMNS OF MICROPARTICLE AMBERLITE XAD-2
    BAUM, RG
    SAETRE, R
    CANTWELL, FF
    [J]. ANALYTICAL CHEMISTRY, 1980, 52 (01) : 15 - 19
  • [6] SOLVENT STRENGTH STUDIES ON POLYSTYRENE-DIVINYLBENZENE COLUMNS
    BOWERS, LD
    PEDIGO, S
    [J]. JOURNAL OF CHROMATOGRAPHY, 1986, 371 : 243 - 251
  • [7] BRANDRUP BA, 1975, POLYM HDB
  • [8] On a theory of the van der Waals adsorption of gases
    Brunauer, S
    Deming, LS
    Deming, WE
    Teller, E
    [J]. JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 1940, 62 : 1723 - 1732
  • [9] ELECTRICAL DOUBLE-LAYER MODELS OF ION-MODIFIED (ION-PAIR) REVERSED-PHASE LIQUID-CHROMATOGRAPHY
    CHEN, JG
    WEBER, SG
    GLAVINA, LL
    CANTWELL, FF
    [J]. JOURNAL OF CHROMATOGRAPHY A, 1993, 656 (1-2) : 549 - 576
  • [10] CHARACTERIZATION OF SOME STYRENE DIVINYLBENZENE SORBENTS FOR REVERSED-PHASE CHROMATOGRAPHY
    COPPI, S
    BETTI, A
    BIGHI, C
    CARTONI, GP
    COCCIOLI, F
    [J]. JOURNAL OF CHROMATOGRAPHY, 1988, 442 : 97 - 103