A high-throughput screen for MscL channel activity and mutational phenotyping

被引：29

作者：

Maurer, JA ^{[1
]}

Dougherty, DA ^{[1
]}

机构：

[1] CALTECH, Div Chem & Chem Engn, Pasadena, CA 91125 USA

来源：

BIOCHIMICA ET BIOPHYSICA ACTA-BIOMEMBRANES | 2001年 / 1514卷 / 02期

关键词：

MscL; mechanosensation; ion channel; fluorescence; gain of function; loss of function;

D O I：

10.1016/S0005-2736(01)00390-X

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

A novel fluorescence-based screen for bacterial mechanosensitive ion-channel activity has been developed. This assay is capable of clearly distinguishing the previously observed gain of function and loss of function phenotypes for the Escherichia coli mechanosensitive channel of large conductance (Ec-MscL). The method modifies Molecular Probes' Live/Dead((R)) BacLight((TM)) bacterial viability assay to monitor MscL channel activity as a function of bacterial survival from osmotic downshock. (C) 2001 Published by Elsevier Science B.V.

引用

页码：165 / 169

页数：5

共 25 条

[1]

Batiza AF, 2000, BIOPHYS J, V78, p473A

[2]

Batiza AF, 1999, BIOPHYS J, V76, pA138

[3] Single residue substitutions that change the gating properties of a mechanosensitive channel in Escherichia coli [J].