Differentiation of rat adipose tissue-derived stem cells into Schwann-like cells in vitro

被引：81

作者：

Jiang, Li ^{[1
,2
]}

Zhu, Jia-Kai ^{[1
,2
]}

Liu, Xiao-Lin ^{[1
]}

Xiang, Peng ^{[2
]}

Hu, Jun ^{[1
]}

Yu, Wei-Hua ^{[2
]}

机构：

[1] Sun Yat Sen Univ, Affiliated Hosp 1, Dept Orthoped & Microsurgery, Guangzhou 510275, Guangdong, Peoples R China

[2] Sun Yat Sen Univ, Ctr Stem cell Biol & Tissue Engn, Guangzhou 510275, Guangdong, Peoples R China

来源：

NEUROREPORT | 2008年 / 19卷 / 10期

关键词：

adipose-derived stem cells; differentiation; microsurgery; Schwann cells;

D O I：

10.1097/WNR.0b013e3283040efc

中图分类号：

Q189 [神经科学];

学科分类号：

071006 [神经生物学];

摘要：

In this study, we explored the competence of adipose-derived stem cells to differentiate into Schwann cells in vitro. Rat adipose-derived stem cells were sequentially treated with various factors beta-mercaptoethanol, all-trans-retinoic acid, followed by a mixture of forskolin, basic fibroblast growth factor, platelet-derived growth factor and heregulin. We found that differentiated adipose-derived stem cells displayed the morphology of Schwarm cells. Western blotting and dual immunofluorescence staining confirmed that they produced proteins characteristic for Schwann cells, including S100 and glial fibrillary acidic protein. Furthermore, differentiated adipose-derived stem cells could enhance neurite outgrowth in coculture with sensory neurons. These results demonstrate that adipose-derived stem cells can differentiate into Schwann-like cells with morphological, phenotypic, and functional characteristics of Schwann cells.

引用

页码：1015 / 1019

页数：5

共 20 条

[1]

In vitro and in vivo differentiation of boundary cap neural crest stem cells into mature Schwann cells [J].