Immobilization of natural products on glass slides by using a photoaffinity reaction and the detection of protein-small-molecule interactions

被引：121

作者：

Kanoh, N

Kumashiro, S

Simizu, S

Kondoh, Y

Hatakeyama, S

Tashiro, H

Osada, H

机构：

[1] RIKEN, Inst Phys & Chem Res, Discovery Res Inst, Antibiot Lab, Wako, Saitama 3510198, Japan

[2] RIKEN, Inst Phys & Chem Res, Adv Engn Ctr, Wako, Saitama 3510198, Japan

来源：

ANGEWANDTE CHEMIE-INTERNATIONAL EDITION | 2003年 / 42卷 / 45期

关键词：

high-throughput screening; immobilization; natural products; photochemistry; protein-ligand interactions;

D O I：

10.1002/anie.200352164

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

Library on a glass slide: A variety of small molecules, including complex natural products, are introduced to glass slides using a photoaffinity reaction (see picture). The small molecules retain the inherent ability to interact with the relevant binding proteins. Importantly, this immobilization procedure does not require a specific functional group, and can be applied for use with a diverse small-molecule library.

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收藏

页码：5584 / 5587

页数：4

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