Development of polymorphic microsatellite loci for North American river otters (Lontra canadensis) and amplification in related Mustelids

被引：38

作者：

Beheler, AS

Fike, JA

Murfitt, LM

Rhodes, OE

Serfass, TS

机构：

[1] Purdue Univ, Dept Forestry & Nat Resources, W Lafayette, IN 47907 USA

[2] Frostburg State Univ, Dept Biol, Frostburg, MD 21532 USA

来源：

MOLECULAR ECOLOGY NOTES | 2004年 / 4卷 / 01期

关键词：

Lontra canadensis; mustelid; microsatellite; river otter;

D O I：

10.1046/j.1471-8286.2003.00564.x

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

In order to complement ecological research on native and reintroduced populations of the North American river otter (Lontra canadensis), we developed 10 polymorphic microsatellite loci that would permit us to evaluate population genetic structure and conduct fecal DNA analyses. The number of alleles per locus ranged from 3 to 14 and observed heterozygosity ranged from 0.23 to 0.68. Preliminary screening revealed that primers were polymorphic in a subset of other Mustelids.

引用

页码：56 / 58

页数：3

共 8 条

[1] Universal linker and ligation procedures for construction of genomic DNA libraries enriched for microsatellites [J].