The structure of importin-β bound to SREBP-2:: Nuclear import of a transcription factor

被引：181

作者：

Lee, SJ

Sekimoto, T

Yamashita, E

Nagoshi, E

Nakagawa, A

Imamoto, N

Yoshimura, M

Sakai, H

Chong, KT

Tsukihara, T

Yoneda, Y

机构：

[1] Osaka Univ, Grad Sch Frontier Biosci, Dept Frontier Biosci, Suita, Osaka 5650871, Japan

[2] Osaka Univ, Inst Prot Res, Suita, Osaka 5650871, Japan

来源：

SCIENCE | 2003年 / 302卷 / 5650期

关键词：

D O I：

10.1126/science.1088372

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

The sterol regulatory element - binding protein 2 (SREBP-2), a nuclear transcription factor that is essential for cholesterol metabolism, enters the nucleus through a direct interaction of its helix-loop-helix leucine zipper domain with importin-beta. We show the crystal structure of importin-beta complexed with the active form of SREBP-2. Importin-beta uses characteristic long helices like a pair of chopsticks to interact with an SREBP-2 dimer. Importin-beta changes its conformation to reveal a pseudo-twofold symmetry on its surface structure so that it can accommodate a symmetric dimer molecule. Importin-beta may use a similar strategy to recognize other dimeric cargoes.

引用

页码：1571 / 1575

页数：5

共 34 条

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