Differentiation of rat bone marrow cells cultured on artificial basement membrane containing extracellular matrix into a liver cell lineage

被引：46

作者：

Okumoto, K ^{[1
]}

Saito, T ^{[1
]}

Hattori, E ^{[1
]}

Ito, J ^{[1
]}

Suzuki, A ^{[1
]}

Misawa, K ^{[1
]}

Ishii, R ^{[1
]}

Karasawa, T ^{[1
]}

Haga, H ^{[1
]}

Sanjo, M ^{[1
]}

Takeda, T ^{[1
]}

Sugahara, K ^{[1
]}

Saito, K ^{[1
]}

Togashi, H ^{[1
]}

Kawata, S ^{[1
]}

机构：

[1] Yamagata Univ, Sch Med, Dept Internal Med & Mol Therapeut, Div Gastroenterol, Yamagata 9909585, Japan

来源：

JOURNAL OF HEPATOLOGY | 2005年 / 43卷 / 01期

基金：

日本学术振兴会;

关键词：

HGF; ECM; liver regeneration; albumin; Musashi-1; transplantaion; stem cell;

D O I：

10.1016/j.jhep.2005.01.037

中图分类号：

R57 [消化系及腹部疾病];

学科分类号：

摘要：

Background/Aims: Bone marrow (BM) cells have been shown to be capable of differentiating into a liver cell lineage in vitro. However, their differentiation and proliferation is poor, and the cell characteristics are poorly understood. Methods: We cultured rat BM cells on an artificial basement membrane containing extracellular matrix (ECM) with hepatocyte growth factor (HGF). The expression of mRNA for liver-specific genes was analyzed by reverse transcription PCR. The expression of albumin and Musashi-1 by cultured cells was analyzed using a fluorescence-activated cell sorter (FACS). The proportions of albumin-positive cells when culture was performed with different concentrations of HGF were analyzed by FACS. Results: On culture day 21, polygonal cells proliferated and formed cell colonies. These cells expressed mRNA for all the liver-specific genes analyzed, and showed heterogeneous differentiation, some cells expressing albumin, others expressing Musashi-1. Albumin-positive differentiated cells were large and rich in intracellular structures, while Musashi-1-positive undifferentiated cells had the opposite characteristics. Culturing cells with higher concentrations of HGF induced an increased proportion of albumin-positive cells. Conclusions: The results suggest that cell culture on an ECM with a high concentration of HGF increases the extent to which BM cells differentiate into a liver cell lineage and proliferate in vitro. (c) 2005 European Association for the Study of the Liver. Published by Elsevier B.V. All rights reserved.

引用

页码：110 / 116

页数：7

共 27 条

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