Enhancing alfalfa conversion efficiencies for sugar recovery and ethanol production by altering lignin composition

被引：81

作者：

Dien, Bruce S. ^{[1
]}

Miller, David J. ^{[2
]}

Hector, Ronald E. ^{[1
]}

Dixon, Richard A. ^{[3
]}

Chen, Fang ^{[3
]}

McCaslin, Mark ^{[4
]}

Reisen, Peter ^{[4
]}

Sarath, Gautam ^{[5
]}

Cotta, Michael A. ^{[1
]}

机构：

[1] ARS, Bioenergy Res Unit, USDA, Natl Ctr Agr Utilizat Res, Peoria, IL 61604 USA

[2] Pioneer HiBred Int Inc, Arlington, WI 53911 USA

[3] Samuel Roberts Noble Fdn Inc, Div Plant Biol, Ardmore, OK 73401 USA

[4] Forage Genet, Nampa, ID 83651 USA

[5] Univ Nebraska, Forage & Bioenergy Res Unit, USDA, ARS, Lincoln, NE 68583 USA

来源：

BIORESOURCE TECHNOLOGY | 2011年 / 102卷 / 11期

关键词：

Biomass; Alfalfa; Bioethanol; Pretreatment; Lignin-modified transgenics; CAFFEIC ACID 3-O-METHYLTRANSFERASE; BIOFUEL PRODUCTION; TRANSGENIC ALFALFA; FUEL ETHANOL; BIOMASS; SACCHARIFICATION; BIOSYNTHESIS; IMPACTS; FERMENTATION; PRETREATMENT;

D O I：

10.1016/j.biortech.2011.03.022

中图分类号：

S2 [农业工程];

学科分类号：

0828 ;

摘要：

Alfalfa (Medicago sativa L) biomass was evaluated for biochemical conversion into ethanol using dilute-acid and ammonia pretreatments. The two alfalfa lines compared were a reduced S-lignin transgenic cultivar generated through down regulation of the caffeic acid O-methyltransferase gene and a wild-type control. Both were harvested at two maturities. All the samples had similar carbohydrate contents including a mean composition of 316 g glucan and 497 g total neutral carbohydrates per kg dry biomass, which corresponds to a theoretic ethanol yield of 3821/ton. Ethanol yields for alfalfa stems pretreated with dilute-acid were significantly impacted by harvest maturity and lignin composition, whereas when pretreated with dilute-ammonia, yield was solely affected by lignin composition. Use of a recombinant xylose-fermenting Saccharomyces strain, for converting the ammonia pretreated alfalfa samples, further increased ethanol yields. Ethanol yields for the xylose-fermenting yeast were 232-278 l/ton and were significantly enhanced for the reduced S lignin cultivars. Published by Elsevier Ltd.

引用

页码：6479 / 6486

页数：8

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