High capacity substrates as a platform for a DNA probe array genotyping assay

被引:13
作者
Fidanza, J [1 ]
Glazer, M
Mutnick, D
McGall, G
Frank, C
机构
[1] Affymetrix Inc, Santa Clara, CA USA
[2] Stanford Univ, Stanford, CA 94305 USA
关键词
D O I
10.1081/NCN-100002329
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Colloidal silica particles were deposited on a glass substrate to produce high-capacity porous supports for high-density DNA probe arrays. Porous surfaces were used to increase the addressable surface area and number of probes available for hybridization. Surfaces derived from 70-100 nm size particles deposited in films from 0.15 to 2 microns thick exhibited excellent performance in light-directed oligonucleotide synthesis. Evaluation of these substrates in a genotyping assay is reported.
引用
收藏
页码:533 / 538
页数:6
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