Molecular organization and functionality of exopolysaccharide gene clusters in lactic acid bacteria

被引：117

作者：

Jolly, L ^{[1
]}

Stingele, F ^{[1
]}

机构：

[1] Nestec Ltd, Nestle Res Ctr, CH-1000 Lausanne 26, Switzerland

来源：

INTERNATIONAL DAIRY JOURNAL | 2001年 / 11卷 / 09期

关键词：

Eps cluster; lactic acid bacteria; exopolysaccharides;

D O I：

10.1016/S0958-6946(01)00117-0

中图分类号：

TS2 [食品工业];

学科分类号：

0832 ;

摘要：

In the dairy industry, microbial exopolysaccharides (EPSs) produced by lactic acid bacteria (LAB) in situ are used to improve the texture of fermented dairy products such as yoghurt or cheese. In addition, it has been suggested that EPSs produced by LAB may confer health benefits. Over the past few years, several studies have been initiated to further understand the genetics and biochemistry of microbial EPS biosynthesis. Eps clusters from different strains of LAB have been identified. In this study, we present the homologies shared by the genes present in the eps clusters from Streptococcus thermophilus, Streptococcus macedonicus, Lactobacillus helveticus, Lactobacillus delbrueckii subsp, bulgaricus and Lactococcus lactis strains. These homologies are striking and imply a common mechanism of EPS biosynthesis. Most interestingly, these clusters contain genes encoding putative glycosyltransferases (GTFs) that can be regarded as a toolbox of enzymes with a large variety of specificities for applications in polysaccharide engineering. (C) 2001 Elsevier Science Ltd. All rights reserved.

引用

页码：733 / 745

页数：13

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