Environmental Sources of Scrapie Prions

被引：47

作者：

Maddison, Ben C. ^{[2
]}

Baker, Claire A. ^{[2
]}

Terry, Linda A. ^{[3
]}

Bellworthy, Susan J. ^{[3
]}

Thorne, Leigh ^{[3
]}

Rees, Helen C. ^{[2
]}

Gough, Kevin C. ^{[1
]}

机构：

[1] Univ Nottingham, Sch Vet Med & Sci, Loughborough LE12 5RD, Leics, England

[2] Univ Leicester, ADAS UK, Dept Biol, Leicester LE1 7RH, Leics, England

[3] Vet Labs Agcy, Addlestone KT15 3NB, Surrey, England

来源：

JOURNAL OF VIROLOGY | 2010年 / 84卷 / 21期

关键词：

CHRONIC WASTING DISEASE; IN-VITRO AMPLIFICATION; INFECTIOUS PRIONS; PROTEIN; BLOOD; URINE; SHEEP; DEER; TRANSMISSION; EXCRETION;

D O I：

10.1128/JVI.01133-10

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

Ovine scrapie and cervine chronic wasting disease show considerable horizontal transmission. Here we report that a scrapie-affected sheep farm has a widespread environmental contamination with prions. Prions were amplified by protein-misfolding cyclic amplification (sPMCA) from seven of nine environmental swab samples taken, including those from metal, plastic, and wooden surfaces. Sheep had been removed from the areas from which the swabs were taken up to 20 days prior to sampling, indicating that prions persist for at least that long. These data implicate inanimate objects as environmental reservoirs for prion infectivity that are likely to contribute to facile disease transmission.

引用

页码：11560 / 11562

页数：3

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