Differential relocation and stability of PML-body components during productive human cytomegalovirus infection: Detailed characterization by live-cell imaging

被引：16

作者：

Dimitropoulou, Panagiota ^{[1
]}

Caswell, Richard ^{[2
]}

McSharry, Brian P. ^{[3
]}

Greaves, Richard F. ^{[4
]}

Spandidos, Demetrios A. ^{[1
]}

Wilkinson, Gavin W. G. ^{[3
]}

Sourvinos, George ^{[1
]}

机构：

[1] Univ Crete, Fac Med, Dept Virol, Iraklion 71003, Crete, Greece

[2] Cardiff Univ, Cardiff Sch Biosci, Cardiff, Wales

[3] Cardiff Univ, Dept Infect Immun & Biochem, Cardiff CF14 4XN, S Glam, Wales

[4] Univ London Imperial Coll Sci Technol & Med, Fac Med, Div Investigat Sci, Dept Virol, London W2 1PG, England

来源：

EUROPEAN JOURNAL OF CELL BIOLOGY | 2010年 / 89卷 / 10期

基金：

英国医学研究理事会; 英国生物技术与生命科学研究理事会; 英国惠康基金;

关键词：

HCMV; IE1-72K; ND10; PML; Sp100; hDaxx; STAT1; STAT2; Condensed chromatin; Live-cell microscopy; EARLY GENE-EXPRESSION; SIMPLEX-VIRUS TYPE-1; PROMYELOCYTIC-LEUKEMIA-PROTEIN; IMMEDIATE-EARLY PROTEINS; LOW-MULTIPLICITY INFECTION; NUCLEAR DOMAIN-10 ND10; MEDIATED REPRESSION; IE1; PROTEIN; TRANSCRIPTIONAL REGULATION; REPLICATION COMPARTMENTS;

D O I：

10.1016/j.ejcb.2010.05.006

中图分类号：

Q2 [细胞生物学];

学科分类号：

071013 [干细胞生物学];

摘要：

In controlling the switch from latency to lytic infection, the immediate early (IE) genes lie at the core of herpesvirus pathogenesis. To image the 72 kDa human cytomegalovirus (HCMV) major IE protein (IE1-72K), a recombinant virus encoding IE1 fused with EGFP was constructed. Using this construct, the IE1-EGFP fusion was detected at ND10 (PML-bodies) within 2 h post infection (p.i.) and the complete disruption of ND10 imaged through to 6 h p.i. HCMV genomes and IE2-86K protein could be detected adjacent to the slowly degradingIE1-72K/ND10 foci. IE1-72K associates with metaphase chromatin, recruiting both PML and STAT2. hDaxx, STAT1 and IE2-86K did not re-locate to metaphase chromatin; the fate of hDaxx is particularly important as this protein contributes to an intrinsic barrier to HCMV infection. While IE1-72K participates in a complex with chromatin, PML, STAT2 and Sp 100,IE1-72K releases hDaxx from ND10 yet does not appear to remain associated with it. (C) 2010 Elsevier GmbH. All rights reserved.

引用

页码：757 / 768

页数：12

共 76 条

[1]

The major immediate-early proteins IE1 and IE2 of human cytomegalovirus colocalize with and disrupt PML-asscciated nuclear bodies at very early times in infected permissive cells [J].