A Novel In Vivo Joint Loading System to Investigate the Effect of Daily Mechanical Load on a Healing Anterior Cruciate Ligament Reconstruction

被引:9
作者
Eng, Mark Stasiak M. [1 ,2 ]
Imhauser, Carl [1 ]
Packer, Jonathan [1 ]
Bedi, Asheesh [1 ]
Brophy, Robert [1 ]
Kovacevic, David [1 ]
Jackson, Kent [1 ]
Deng, Xiang-Hua [1 ]
Rodeo, Scott [1 ]
Torzilli, Peter [1 ,2 ]
机构
[1] Hosp Special Surg, Lab Soft Tissue Res, New York, NY 10021 USA
[2] CUNY City Coll, Dept Biomed Engn, New York, NY 10031 USA
来源
JOURNAL OF MEDICAL DEVICES-TRANSACTIONS OF THE ASME | 2010年 / 4卷 / 01期
关键词
tendon; bone; healing; ACL; in vivo; cyclic strain; mechanical load; BONE TUNNEL; COMPOSITIONAL PROPERTIES; PATELLAR TENDON; EXPRESSION; INSERTION; GRAFT;
D O I
10.1115/1.4001158
中图分类号
R318 [生物医学工程];
学科分类号
100103 [病原生物学];
摘要
We designed and validated a novel knee joint fixation/distraction system to study tendon to-bone healing in an in vivo rat model of anterior cruciate ligament (A CL) reconstruction. The system uses an external fixator to apply a cyclic distraction of the knee joint while monitoring the resultant force developed across the joint, thus providing a temporal indication of structural changes during the healing process of the hone-tendon-bone reconstruction. The validation was performed using an optical kinematic tracking system to determine the local displacement of the knee. The average system compliance was determined to be 42.4 +/- 8.8 mu m/N with a coefficient of variation of 20.7%. The compliance was used to obtain a best fit correction factor which brought the total root mean square error of knee joint distraction to within 179 pm (16.1%) of the applied distraction. We performed a pilot study using 15 rats that had ACL reconstructions using a flexor digitorum longus tendon autograft and found that the animals tolerated the indwelling fixator and daily anesthesia over a 10 day loading protocol. Our knee joint fixation/distraction system provides a valuable tool to study how mechanical stimuli affect in vivo bone-tendon-bone healing. [DOI: 10.1115/1.4001158]
引用
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页数:7
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