Herpes simplex virus DNA packaging sequences adopt novel structures that are specifically recognized by a component of the cleavage and packaging machinery

被引:83
作者
Adelman, K [1 ]
Salmon, B [1 ]
Baines, JD [1 ]
机构
[1] Cornell Univ, Dept Microbiol & Immunol, Ithaca, NY 14853 USA
关键词
D O I
10.1073/pnas.061555698
中图分类号
O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];
学科分类号
07 ; 0710 ; 09 ;
摘要
The product of the herpes simplex virus type 1 U(L)28 gene is essential for cleavage of concatemeric viral DNA into genome-length units and packaging of this DNA into viral procapsids. To address the role of UL28 in this process, purified U(L)28 protein was assayed for the ability to recognize conserved herpesvirus DNA packaging sequences. We report that DNA fragments containing the pac1 DNA packaging motif can be induced by heat treatment to adopt novel DNA conformations that migrate faster than the corresponding duplex in nondenaturing gels, Surprisingly, these novel DNA structures are high-affinity substrates for UL28 protein binding, whereas double-stranded DNA of identical sequence composition is not recognized by U(L)28 protein. We demonstrate that only one strand of the pad motif is responsible for the formation of novel DNA structures that are bound tightly and specifically by UL28 protein, To determine the relevance of the observed U(L)28 protein-pad interaction to the cleavage and packaging process, we have analyzed the binding affinity of U(L)28 protein for pad mutants previously shown to be deficient in cleavage and packaging in vivo. Each of the pac1 mutants exhibited a decrease in DNA binding by U(L)28 protein that correlated directly with the reported reduction in cleavage and packaging efficiency, thereby supporting a role for the U(L)28 protein-pad interaction in vivo. These data therefore suggest that the formation of novel DNA structures by the pad motif confers added specificity on recognition of DNA packaging sequences by the U(L)28-encoded component of the herpesvirus cleavage and packaging machinery.
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页码:3086 / 3091
页数:6
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