Clathrate nanostructures for mass spectrometry

被引:389
作者
Northen, Trent R.
Yanes, Oscar
Northen, Michael T.
Marrinucci, Dena
Uritboonthai, Winnie
Apon, Junefredo
Golledge, Stephen L.
Nordstrom, Anders
Siuzdak, Gary
机构
[1] Scripps Res Inst, Dept Mol Biol, La Jolla, CA 92037 USA
[2] Scripps Res Inst, Scripps Ctr Mass Spectrometry, La Jolla, CA 92037 USA
[3] Scripps Res Inst, Dept Cell Biol, La Jolla, CA 92037 USA
[4] Univ Calif Santa Barbara, Dept Mat, Santa Barbara, CA 93106 USA
[5] Univ Oregon, CAMCOR Surface Anal Facil, Eugene, OR 97403 USA
基金
美国国家卫生研究院; 美国国家科学基金会;
关键词
D O I
10.1038/nature06195
中图分类号
O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];
学科分类号
07 ; 0710 ; 09 ;
摘要
The ability of mass spectrometry to generate intact biomolecular ions efficiently in the gas phase has led to its widespread application in metabolomics(1), proteomics(2), biological imaging(3), biomarker discovery(4) and clinical assays (namely neonatal screens(5)). Matrix-assisted laser desorption/ionization(6,7) (MALDI) and electrospray ionization(8) have been at the forefront of these developments. However, matrix application complicates the use of MALDI for cellular, tissue, biofluid and microarray analysis and can limit the spatial resolution because of the matrix crystal size(9) (typically more than 10 mu m), sensitivity and detection of small compounds (less than 500 Da). Secondary-ion mass spectrometry(10) has extremely high lateral resolution (100 nm) and has found biological applications(11,12) although the energetic desorption/ionization is a limitation owing to molecular fragmentation. Here we introduce nanostructure- initiator mass spectrometry (NIMS), a tool for spatially defined mass analysis. NIMS uses 'initiator' molecules trapped in nanostructured surfaces or 'clathrates' to release and ionize intact molecules adsorbed on the surface. This surface responds to both ion and laser irradiation. The lateral resolution (ion-NIMS about 150 nm), sensitivity, matrix-free and reduced fragmentation of NIMS allows direct characterization of peptide microarrays, direct mass analysis of single cells, tissue imaging, and direct characterization of blood and urine.
引用
收藏
页码:1033 / U3
页数:5
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