Circadian-regulated transcription of the psbD light-responsive promoter in wheat chloroplasts

被引:49
作者
Nakahira, Y [1 ]
Baba, K [1 ]
Yoneda, A [1 ]
Shiina, T [1 ]
Toyoshima, Y [1 ]
机构
[1] Kyoto Univ, Grad Sch Human & Environm Studies, Sakyo Ku, Kyoto 6068501, Japan
关键词
D O I
10.1104/pp.118.3.1079
中图分类号
Q94 [植物学];
学科分类号
071001 ;
摘要
The level of mRNAs derived from the plastid-encoded psbD light-responsive promoter (LRP) is controlled by a circadian clock(s) in wheat ( Triticum aestivum). The circadian oscillations in the psbD LRP mRNA level persisted for at least three cycles in continuous light and for one cycle in continuous dark, with maxima in subjective morning and minima in subjective early night. In vitro transcription in chloroplast extracts revealed that the circadian cycles in the psbD LRP mRNA level were dominantly attributed to the circadian-regulated transcription of the psbD LRP. The effects of various mutations introduced into the promoter region on the psbD LRP activity in vitro suggest the existence of two positive elements located between -54 and -36, which generally enhance the transcription activity, and an anomalous core promoter structure lacking the functional "-35" element, which plays a crucial role in the circadian fluctuation and light dependency of psbD LRP transcription activity.
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页码:1079 / 1088
页数:10
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