Determination of alternariol in tomato paste using solid phase extraction and high-performance liquid chromatography with fluorescence detection

被引：20

作者：

Fente, CA ^{[1
]}

Jaimez, J ^{[1
]}

Vázquez, BI ^{[1
]}

Franco, CM ^{[1
]}

Cepeda, A ^{[1
]}

机构：

[1] Univ Santiago de Compostela, Fac Vet, Dpto Quim Analit Nutr & Bromatol, Lab Higiene & Inspecc Alimentos, E-27002 Lugo, Spain

来源：

ANALYST | 1998年 / 123卷 / 11期

关键词：

D O I：

10.1039/a804587i

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

Alternaria spp. produce a wide variety of toxic metabolites with different chemical structures. Tomato products have been considered a likely source of Alternaria toxins in the human diet because Alternaria is an important spoilage mold of tomatoes. A new method for the determination of these mycotoxins in tomato paste, involving solid phase cartridges for extraction before HPLC fluorescence detection with a reversed phase column and isocratic elution, was developed. The method was demonstrated to be linear in the range 5.2-196 ppb of alternariol (AOH) in tomato paste. Good recoveries were obtained for AOH at all levels assayed (minimum 77.2%). The detection limit of the AOH toxin in real samples of tomato paste was low, 1.93 ppb, The precision of the method was demonstrated with a good repeatability (RSD = 2.98%) and reproducibility (RSD = 9.35%).

引用

页码：2277 / 2280

页数：4

共 21 条

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