Hypomethylated and hypermethylated profiles of H19DMR are associated with the aberrant imprinting of IGF2 and H19 in human hepatocellular carcinoma

被引：61

作者：

Wu, Jing ^{[1
,2
]}

Qin, Yang ^{[1
,2
]}

Li, Bo ^{[3
]}

He, Wen-zhi ^{[1
]}

Sun, Zhi-lin ^{[1
]}

机构：

[1] W China Med Ctr, Sch Preclin & Forens Med, Dept Biochem & Mol Biol, Chengdu 610041, Sichuan Prov, Peoples R China

[2] Sichuan Univ, State Key Lab Biotherapy, Chengdu 610041, Sichuan Prov, Peoples R China

[3] W China Med Ctr, Dept Gen Surg, Chengdu 610041, Sichuan Prov, Peoples R China

来源：

GENOMICS | 2008年 / 91卷 / 05期

基金：

中国国家自然科学基金;

关键词：

methylation; imprinting; IGF2; H19; human hepatocellular carcinoma; liver;

D O I：

10.1016/j.ygeno.2008.01.007

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

In this study, 39 human hepatocellular carcinoma (HCC) tissues and 7 normal adult liver tissues were screened for heterozygous polymorphisms in IGF2, H19, and the differentially methylated region of H19 (H19DMR) using PCR-RFLP and PCR sequencing. The imprinting of IGF2 and H19 was examined by RT-PCR-RFLP, while the methylation profile of H19DMR was detected by bisulfite sequencing from every informative sample. Of the informative HCC samples 47.06% (8 of 17) demonstrated a gain of imprinting of IGF2, and 21.74% (5 of 23) of the informative HCC samples demonstrated a loss of imprinting of H19. Interestingly, we found three methylation profiles for H19DMR in the informative HCC samples: hyper-, medium-, and hypomethylated profiles. Furthermore, the hypomethylated and hypermethylated profiles were immediately associated with aberrant imprinting of IGF2 and H19. (C) 2008 Elsevier Inc. All rights reserved.

引用

页码：443 / 450

页数：8

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