In vitro correlate of immunity in a rabbit model of inhalational anthrax

被引：230

作者：

Pitt, MLM ^{[1
]}

Little, SF ^{[1
]}

Ivins, BE ^{[1
]}

Fellows, P ^{[1
]}

Barth, J ^{[1
]}

Hewetson, J ^{[1
]}

Gibbs, P ^{[1
]}

Dertzbaugh, M ^{[1
]}

Friedlander, AM ^{[1
]}

机构：

[1] USA, Med Res Inst Infect Dis, Frederick, MD 21702 USA

来源：

VACCINE | 2001年 / 19卷 / 32期

关键词：

anthrax; Bacillus anthracis; serological correlate;

D O I：

10.1016/S0264-410X(01)00234-1

中图分类号：

R392 [医学免疫学]; Q939.91 [免疫学];

学科分类号：

100102 ;

摘要：

A serological correlate of vaccine-induced immunity was identified in the rabbit model of inhalational anthrax. Animals were inoculated intramuscularly at 0 and 4 weeks with varying doses of Anthrax Vaccine Adsorbed (AVA) ranging from a human dose to a 1:256 dilution in phosphate-buffered saline (PBS). At 6 and 10 weeks, both the quantitative anti-protective antigen (PA) I-G ELISA and the toxin-neutralizing antibody (TNA) assays were used to measure antibody levels to PA. Rabbits were aerosol-challenged at 10 weeks with a lethal dose (84-133 LD50) of Bacillus anthracis spores. All the rabbits that received the undiluted and 1:4 dilution of vaccine survived, whereas those receiving the higher dilutions of vaccine (1: 16, 1:64 and 1:256) had deaths in their groups. Results showed that antibody levels to PA at both 6 and 10 weeks were significant (P < 0.0001) predictors of survival. Published by Elsevier Science Ltd.

引用

页码：4768 / 4773

页数：6

共 40 条

[1] Vaccination against anthrax with attenuated recombinant strains of Bacillus anthracis that produce protective antigen [J].