Extensive maternal DNA hypomethylation in the endosperm of Zea mays

被引：77

作者：

Lauria, M

Rupe, M

Guo, M

Kranz, E

Pirona, R

Viotti, A

Lund, G ^{[1
]}

机构：

[1] Royal Vet & Agr Univ, Dept Plant Biol, Plant Biochem Lab, DK-1871 Frederiksberg C, Denmark

[2] Pioneer HiBred Int Inc, Johnston, IA 50131 USA

[3] Univ Hamburg, Inst Allgemeine Bot, D-22609 Hamburg, Germany

[4] Ist Sperimentale Cerealicoltura, Sec Bergamo, I-24126 Bergamo, Italy

[5] CNR, Ist Biol & Biotecnol Agraria, I-20133 Milan, Italy

来源：

PLANT CELL | 2004年 / 16卷 / 02期

关键词：

D O I：

10.1105/tpc.017780

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

A PCR-based genomic scan has been undertaken to estimate the extent and ratio of maternally versus paternally methylated DNA regions in endosperm, embryo, and leaf of Zea mays (maize). Analysis of several inbred lines and their reciprocal crosses identified a large number of conserved, differentially methylated DNA regions (DMRs) that were specific to the endosperm. DMRs were hypomethylated at specific methylation-sensitive restriction sites upon maternal transmission, whereas upon paternal transmission, the methylation levels were similar to those observed in embryo and leaf. Maternal hypomethylation was extensive and offers a likely explanation for the 13% reduction in methyl-cytosine content of the endosperm compared with leaf tissue. DMRs showed identity to expressed genic regions, were observed early after fertilization, and maintained at a later stage of endosperm development. The implications of extensive maternal hypomethylation with respect to endosperm development and epigenetic reprogramming will be discussed.

引用

页码：510 / 522

页数：13

共 59 条

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