Neurofilament protein expression in the geniculostriate pathway of a New World monkey (Callithrix jacchus)

We examined the expression profile of nonphosphorylated neurofilament protein in the dorsal lateral geniculate nucleus (LGN) and striate cortex (VI) of a New World simian, the marmoset monkey, using the monoclonal antibody SMI-32. The overall distribution of neurofilament protein in the marmoset resembled that previously described in Old World monkeys. While immunostained neurones were observed throughout the LGN, there were clear laminar differences in terms of both cellular and neuropil labelling. Neurones in the magnocellular layer cells stained more densely than those in the parvocellular layers. The marmoset's well-defined koniocellular layers showed an overall light stain of both neurones and neuropil. In VI, densely stained pyramidal cells and heavy neuropil label were observed in the two sublayers that send projections to the middle temporal area (MT): a supragranular band located in layer 3C (Brodmann's layer 4B) and an infragramilar band located near the top of layer 6. More lightly stained, small pyramidal cells were also found in layer Ma. Accordingly, in both New World and Old World monkeys the expression of neurofilament protein is correlated with specific functional subdivisions of the geniculocortical pathway. In particular, projection neurones associated with fast-conducting pathways to the extrastriate 'dorsal stream' appear to contain higher levels of this protein.