RuBi-Glutamate: two-photon and visible-light photoactivation of neurons and dendritic spines

被引：145

作者：

Fino, Elodie ^{[1
]}

Araya, Roberto ^{[1
]}

Peterka, Darcy S. ^{[1
]}

Salierno, Marcelo ^{[2
]}

Etchenique, Roberto ^{[2
]}

Yuste, Rafael ^{[1
]}

机构：

[1] Columbia Univ, Dept Biol Sci, Howard Hughes Med Inst, New York, NY 10027 USA

[2] Univ Buenos Aires, Fac Ciencias Exactas & Nat, Dept Quim Inorgan Analit & Quim Fis, Buenos Aires, DF, Argentina

来源：

FRONTIERS IN NEURAL CIRCUITS | 2009年 / 3卷

关键词：

uncaging; Ruthenium; bipyridine; RuBi-GABA; SYNAPTIC-TRANSMISSION; PHOTOSTIMULATION; RECEPTORS;

D O I：

10.3389/neuro.04.002.2009

中图分类号：

Q189 [神经科学];

学科分类号：

071006 [神经生物学];

摘要：

We describe neurobiological applications of RuBi-Glutamate, a novel caged-glutamate compound based on ruthenium photochemistry. RuBi-Glutamate can be excited with visible wavelengths and releases glutamate after one- or two-photon excitation. It has high quantum efficiency and can be used at low concentrations, partly avoiding the blockade of GABAergic transmission present with other caged compounds. Two-photon uncaging of RuBi-Glutamate has a high spatial resolution and generates excitatory responses in individual dendritic spines with physiological kinetics. With laser beam multiplexing, two-photon RuBi-Glutamate uncaging can also be used to depolarize and fire pyramidal neurons with single-cell resolution. RuBi-Glutamate therefore enables the photoactivation of neuronal dendrites and circuits with visible or two-photon light sources, achieving single cell, or even single spine, precision.

引用

页数：9

共 26 条

[1]

The spine neck filters membrane potentials [J].