Association of mesenchymal stem cells and osteoblasts for bone repair

被引：7

作者：

Santos, Thiago de Santana ^{[1
]}

Flores Abuna, Rodrigo Paolo ^{[1
]}

Lopes, Helena Bacha ^{[1
]}

Goncalves de Almeida, Adriana Luisa ^{[1
]}

Beloti, Marcio Mateus ^{[1
]}

Rosa, Adalberto Luiz ^{[1
,2
]}

机构：

[1] Univ Sao Paulo, Cell Culture Lab, Sch Dent Ribeirao Preto, BR-14040904 Ribeirao Preto, SP, Brazil

[2] Univ Sao Paulo, Dept Oral & Maxillofacial Surg & Periodontol, Sch Dent Ribeirao Preto, BR-14040904 Ribeirao Preto, SP, Brazil

来源：

REGENERATIVE MEDICINE | 2015年 / 10卷 / 02期

基金：

巴西圣保罗研究基金会; 瑞典研究理事会;

关键词：

bone marrow; bone repair; cell therapy; mesenchymal stem cell; osteoblast; REGENERATION; SCAFFOLDS; GRAFT; DEFECTS;

D O I：

10.2217/RME.14.75

中图分类号：

Q813 [细胞工程];

学科分类号：

100113 [医学细胞生物学];

摘要：

Aim: We tested the hypothesis that the association of bone marrow mesenchymal stem cells (MSCs) and osteoblasts (OBs) optimize bone repair. Materials & Methods: MSCs were cultured in growth or osteogenic medium and seeded into gelatin sponge prior to implantation. Defects were created into rat calvariae and implanted with gelatin sponge without cells, with MSCs, with OBs and with association of MSCs and OBs. Histological analysis and micro-CT-based histomorphometry were carried out after 4 weeks. Results: Increased bone formation was observed in defects treated with cells and bone volume was greater in defects treated with either OBs or MSCs/OBs. Conclusion: Association of MSCs and OBs did not increase the process of bone repair compared with cell-based therapy using either MSCs or OBs alone.

引用

页码：127 / 133

页数：7

共 26 条

[1]

Evaluation of early healing events around mesenchymal stem cell-seeded collagen-glycosaminoglycan scaffold. An experimental study in Wistar rats [J].

Alhag M. ;

Farrell E. ;

Toner M. ;

Claffey N. ;

Lee T.C. ;

O'Brien F. .

Oral and Maxillofacial Surgery, 2011, 15 (1) :31-39

[2]

Beloti MM, 2012, TISSUE ENG PT A, V18, P999, DOI [10.1089/ten.tea.2011.0405, 10.1089/ten.TEA.2011.0405]

[3]

Seeding Osteoblastic Cells into a Macroporous Biodegradable CaP/PLGA Scaffold by a Centrifugal Force [J].

Beloti, Marcio Mateus ;

De Oliveira, Paulo Tambasco ;

Perri De Carvalho, Paulo Sergio ;

Rosa, Adalberto Luiz .

JOURNAL OF BIOMATERIALS APPLICATIONS, 2009, 23 (06) :481-495

[4]

Guidelines for Assessment of Bone Microstructure in Rodents Using Micro-Computed Tomography [J].

Bouxsein, Mary L. ;

Boyd, Stephen K. ;

Christiansen, Blaine A. ;

Guldberg, Robert E. ;

Jepsen, Karl J. ;

Mueller, Ralph .

JOURNAL OF BONE AND MINERAL RESEARCH, 2010, 25 (07) :1468-1486

[5]

Pre-culture period of mesenchymal stem cells in osteogenic media influences their in vivo bone forming potential [J].

Castano-Izquierdo, Harold ;

Alvarez-Barreto, Jose ;

van den Dolder, Juliette ;

Jansen, John A. ;

Mikos, Antonios G. ;

Sikavitsas, Vassilios I. .

JOURNAL OF BIOMEDICAL MATERIALS RESEARCH PART A, 2007, 82A (01) :129-138

[6]

BONE-GRAFT AND BONE-GRAFT SUBSTITUTES - A REVIEW OF CURRENT TECHNOLOGY AND APPLICATIONS [J].

DAMIEN, CJ ;

PARSONS, JR .

JOURNAL OF APPLIED BIOMATERIALS, 1991, 2 (03) :187-208

[7]

Gazdag, 1995, J Am Acad Orthop Surg, V3, P1

[8]

CURRENT CONCEPTS IN BONE HEALING - REVIEW OF THE LITERATURE [J].

GREENBAUM, MA ;

KANAT, IO .

JOURNAL OF THE AMERICAN PODIATRIC MEDICAL ASSOCIATION, 1993, 83 (03) :123-129

[9]

What should be the characteristics of the ideal bone graft substitute? Combining scaffolds with growth factors and/or stem cells [J].

Janicki, Patricia ;

Schmidmaier, Gerhard .

INJURY-INTERNATIONAL JOURNAL OF THE CARE OF THE INJURED, 2011, 42 :S77-S81

[10]

A multi-center, randomized, clinical study to compare the effect and safety of autologous cultured osteoblast(Ossron™) injection to treat fractures [J].

Kim, Seok-Jung ;

Shin, Yong-Woon ;

Yang, Kyu-Hyun ;

Kim, Sang-Bum ;

Yoo, Moon-Jib ;

Han, Suk-Ku ;

Im, Soo-Ah ;

Won, Yoo-Dong ;

Sung, Yerl-Bo ;

Jeon, Taek-Soo ;

Chang, Cheong-Ho ;

Jang, Jae-Deog ;

Lee, Sae-Bom ;

Kim, Hyun-Cho ;

Lee, Soo-Young .

BMC MUSCULOSKELETAL DISORDERS, 2009, 10

← 1 2 3 →