Autoregulation of the pTF-FC2 proteic poison-antidote plasmid addiction system (pas) is essential for plasmid stabilization

被引：17

作者：

Smith, ASG ^{[1
]}

Rawlings, DE ^{[1
]}

机构：

[1] Univ Cape Town, Dept Microbiol, ZA-7700 Rondebosch, South Africa

来源：

JOURNAL OF BACTERIOLOGY | 1998年 / 180卷 / 20期

关键词：

D O I：

10.1128/JB.180.20.5463-5465.1998

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

The pasABC genes of the proteic plasmid addiction system of broad-host-range plasmid pTF-FC2 were autoregulated. The PasA antidote was able to repress the operon 25-fold on its own, and repression was increased to 100-fold when the PasB toxin was also present. Autoregulation appears to be an essential requirement for pas-mediated plasmid stabilization because when the pas genes were placed behind the isopropyl-beta-D-thiogalactopyranoside (IPTG)-regulated tac promoter, they were unable to stabilize a heterologous test plasmid.

引用

页码：5463 / 5465

页数：3

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