Dormancy-associated gene expression in pea axillary buds.

被引:108
作者
Stafstrom, JP [1 ]
Ripley, BD [1 ]
Devitt, ML [1 ]
Drake, B [1 ]
机构
[1] No Illinois Univ, Dept Biol Sci, Ctr Plant Mol Biol, De Kalb, IL 60115 USA
关键词
apical dominance; axillary bud; bud development; dormancy (bud); Pisum (bud dormancy);
D O I
10.1007/s004250050354
中图分类号
Q94 [植物学];
学科分类号
071001 ;
摘要
Pea (Pisum sativum L. cv. Alaska) axillary buds can be stimulated to cycle between dormant and growing states. Dormant buds synthesize unique proteins and are as metabolically active as growing buds. Two cDNAs, PsDRM1 and PsDRM2, were isolated from a dormant bud library. The deduced amino acid sequence of PsDRM1 (111 residues) is 75% identical to that of an auxin-repressed strawberry clone. PsDRM2 encodes a putative protein containing 129 residues, which includes II repeats of the sequence [G]-GGGY[H][N] (the bracketed residues may be absent). PsDRM2 is related to cold- and ABA-stimulated clones from alfalfa. Decapitating the terminal bud rapidly stimulates dormant axillary buds to begin growing. The abundance of PsDRM1 mRNA in axillary buds declines 20-fold within 6 h of decapitation; it quickly reaccumulates when buds become dormant again. The level of PsDRM2 mRNA is about three fold lower in growing buds than in dormant buds. Expression of PsDRM1 is enhanced in other non-growing organs (roots >>root apices; fully-elongated stems >elongating stems), and thus is an excellent "dormancy" marker. In contrast, PsDRM2 expression is not dormancy-associated in other organs.
引用
收藏
页码:547 / 552
页数:6
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