Cytotoxicity and potential antiviral evaluation of violacein produced by Chromobacterium violaceum

被引:151
作者
Andrighetti-Fröhner, CR
Antonio, RV
Creczynski-Pasa, TB
Barardi, CRM
Simoes, CMO
机构
[1] Univ Fed Santa Catarina, Lab Virol Aplicada, Dept Ciencias Farmaceut, Florianopolis, SC, Brazil
[2] Univ Fed Santa Catarina, Dept Microbiol & Parasitol, Florianopolis, SC, Brazil
[3] Univ Fed Santa Catarina, Lab Bioquim, Dept Bioquim, Florianopolis, SC, Brazil
[4] Univ Fed Santa Catarina, Lab Bioenerget & Comunicacao Celular, Florianopolis, SC, Brazil
来源
MEMORIAS DO INSTITUTO OSWALDO CRUZ | 2003年 / 98卷 / 06期
关键词
violacein; cytotoxicity; antiviral; MTT assay; herpes simplex virus type 1; poliovirus; rotavirus; hepatitis A virus; adenovirus;
D O I
10.1590/S0074-02762003000600023
中图分类号
R38 [医学寄生虫学]; Q [生物科学];
学科分类号
07 ; 0710 ; 09 ; 100103 ;
摘要
Natural products are an inexhaustible source of compounds with promising pharmacological activities including antiviral action. Violacein, the major pigment produced by Chromobacterium violaceum, has been shown to have antibiotic, antitumoral and anti-Trypanosoma cruzi activities. The goal of the present work was to evaluate the cytotoxicity of violacein and also its potential antiviral properties. The cytotoxicity of violacein was investigated by three methods: cell morphology evaluation by inverted light microscopy and cell viability tests using the Trypan blue dye exclusion method and the MTT assay. The cytotoxic concentration values which cause destruction in 50% of the monolayer cells (CC50) were different depending on the sensitivity of the method. CC50 values were greater than or equal to2.07 +/- 0.08 muM for FRhK-4 cells: greater than or equal to2.23 +/- 0.11 muM for Vero cells; greater than or equal to2.54 +/- 0.18 muM for MA104 cells; and greater than or equal to2.70 +/- 0.20 muM for HEp-2 cells. Violacein showed no cytopathic inhibition of the following viruses: herpes simplex virus type 1 (HSV-1) strain 29-R/acyclovir resistant, hepatitis A virus (strains HM175 and HAF-203) and adenovirus type 5 nor did it show any antiviral activity in the MTT assay. However violacein did show a weak inhibition of viral replication: 1.42 +/- 0.68%, 14.48 +/- 5.06% and 21.47 +/- 3.74% for HSV-1 (strain KOS); 5.96 +/- 2.51%, 8.75 +/- 3.08% and 17.75 +/- 5.19% for HSV-1 (strain ATCC/VR-733); 5.13 +/- 2.38%, 8.18 +/- 1.11% and 8.51 +/- 1.94% for poliovirus type 2; 8.30 +/- 4.24%; 13.33 +/- 4.66% and 24.27 +/- 2.18% for simian rotavirus SA11, at 0.312, 0.625 and 1.250 mM, respectively, when measured by the MTT assay.
引用
收藏
页码:843 / 848
页数:6
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