A new amperometric biosensor for fructose using a carbon paste electrode modified with silica gel coated with Meldola's Blue and fructose 5-dehydrogenase

被引：34

作者：

Garcia, CAB ^{[1
]}

Neto, GD ^{[1
]}

Kubota, LT ^{[1
]}

Grandin, LA ^{[1
]}

机构：

[1] UNICAMP,INST QUIM,BR-13083 CAMPINAS,SP,BRAZIL

来源：

JOURNAL OF ELECTROANALYTICAL CHEMISTRY | 1996年 / 418卷 / 1-2期

基金：

巴西圣保罗研究基金会;

关键词：

biosensors; fructose; Meldola's Blue mediator; modified silica gel; 5-dehydrogenase;

D O I：

10.1016/S0022-0728(96)04775-4

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

A carbon paste modified with silica gel coated with Meldola's Blue and fructose 5-dehydrogenase was used to construct a new biosensor for fructose. An efficient electron transfer mediation from the reduced enzyme to the carbon paste electrode was verified, at a low applied potential of 20mV vs. SCE, where interfering reactions are minimized. A linear response range for 0.1 to 0.8 mmol(-1) fructose was adjusted by the equation I=0.0150(+/-0.006)+0.618(+/-0.011) [fructose], with a correlation coefficient of 0.999 and an rsd of 0.68% for n=7. The biosensor was used to determine fructose in sweets and fruit jellies and the results present good recovery, higher than 96% in both cases. A stable response is observed over two months, when stored in a refrigerator, or for 300 measurements in continuous use.

引用

页码：147 / 151

页数：5

共 34 条

[1] A SIMPLE AND RAPID COLORIMETRIC METHOD FOR LACTOSE DETERMINATION IN MILK [J].