The antimicrobial efficacy of Fructus mume extract on orthodontic bracket: A monospecies-biofilm model study in vitro

被引:23
作者
Chen, Yong [1 ]
Wong, Ricky W. K. [1 ]
Seneviratne, C. J. [2 ]
Haegg, Urban [1 ]
Mcgrath, Colman [3 ]
Samaranayake, L. P. [2 ]
Kao, Richard [4 ]
机构
[1] Univ Hong Kong, Fac Dent, Dept Orthodont, Hong Kong, Hong Kong, Peoples R China
[2] Univ Hong Kong, Fac Dent, Dept Oral Biosci, Hong Kong, Hong Kong, Peoples R China
[3] Univ Hong Kong, Fac Dent, Dept Dent Publ Hlth, Hong Kong, Hong Kong, Peoples R China
[4] Univ Hong Kong, Li Ka Shing Fac Med, Dept Microbiol, Hong Kong, Hong Kong, Peoples R China
关键词
Antimicrobial; Fructus mume; Orthodontic; STREPTOCOCCUS-MUTANS LEVELS; ESSENTIAL OIL; CITRIC-ACID; CHLORHEXIDINE; PLAQUE; MOUTHRINSE; GINGIVITIS; CARIES;
D O I
10.1016/j.archoralbio.2010.08.006
中图分类号
R78 [口腔科学];
学科分类号
100302 [口腔临床医学];
摘要
Objective: The aim of this study was to evaluate the antimicrobial efficacy of Traditional Chinese Medicine Fructus mume on a monospecies-biofilm model established on orthodontic brackets in vitro. Materials and methods: The antimicrobial effect of Fructus mume aqueous extract on the planktonic Streptococcus mutans (S. mutans) was tested by microdilution method (MIC). The cell viability of S. mutans biofilm on Damon3 MX bracket (Ormco, USA) after exposed to Fructus mume extract was quantified by XTT reduction assay. Visualization of the samples was performed by fluorescence microscope and confocal laser scanning microscopy (CLSM). Results: HPLC analysis revealed that the main compounds of Fructus mume are organic acids. The MIC of Fructus mume extract on the planktonic S. mutans was 50 mg/mL. The optical density (OD) values, measured by XTT reduction assay from S. mutans biofilms after 1-min exposure to different test agents, demonstrated that the cell viability of S. mutans biofilms exposed to 250 mg/mL Fructus mume extract < BHI (-) (p < 0.01). Microscope image showed that Fructus mume extract obviously increased the amount of dead bacteria on the surface of bracket. Conclusion: Fructus mume extract showed antimicrobial effect on S. mutans biofilm on orthodontic bracket in vitro which may indicate its potential use as an oral antimicrobial agent for orthodontic patients. (C) 2010 Elsevier Ltd. All rights reserved.
引用
收藏
页码:16 / 21
页数:6
相关论文
共 31 条
[1]
Clinical effects of chlorhexidine mouthwashes on patients undergoing orthodontic treatment [J].
Anderson, GB ;
Bowden, J ;
Morrison, EC ;
Caffesse, RG .
AMERICAN JOURNAL OF ORTHODONTICS AND DENTOFACIAL ORTHOPEDICS, 1997, 111 (06) :606-612
[2]
Anti-plaque agents in the prevention of biofilm-associated oral diseases [J].
Baehni, PC ;
Takeuchi, Y .
ORAL DISEASES, 2003, 9 :23-29
[3]
The efficacy of an essential oil antiseptic mouthrinse vs. dental floss in controlling interproximal gingivitis - A comparative study [J].
Bauroth, K ;
Charles, CH ;
Mankodi, SM ;
Simmons, K ;
Zhao, Q ;
Kumar, LD .
JOURNAL OF THE AMERICAN DENTAL ASSOCIATION, 2003, 134 (03) :359-365
[4]
PERIODONTAL HEALING FOLLOWING GUIDED TISSUE REGENERATION WITH CITRIC-ACID AND FIBRONECTIN APPLICATION [J].
CAFFESSE, RG ;
NASJLETI, CE ;
ANDERSON, GB ;
LOPATIN, DE ;
SMITH, BA ;
MORRISON, EC .
JOURNAL OF PERIODONTOLOGY, 1991, 62 (01) :21-29
[5]
Plant products as antimicrobial agents [J].
Cowan, MM .
CLINICAL MICROBIOLOGY REVIEWS, 1999, 12 (04) :564-+
[6]
ANTIBACTERIAL EFFECT OF CITRIC-ACID TREATMENT OF PERIODONTALLY DISEASED ROOT SURFACES INVITRO [J].
DALY, CG .
JOURNAL OF CLINICAL PERIODONTOLOGY, 1982, 9 (05) :386-392
[7]
Dawes Colin, 2003, J Can Dent Assoc, V69, P722
[8]
CLINICAL AND MICROBIOLOGICAL EFFECTS OF FIXED ORTHODONTIC APPLIANCES [J].
DIAMANTIKIPIOTI, A ;
GUSBERTI, FA ;
LANG, NP .
JOURNAL OF CLINICAL PERIODONTOLOGY, 1987, 14 (06) :326-333
[9]
Effect of time on the remineralisation of enamel by synthetic saliva after citric acid erosion [J].
Eisenburger, M ;
Addy, M ;
Hughes, JA ;
Shellis, RP .
CARIES RESEARCH, 2001, 35 (03) :211-215
[10]
POTENTIAL EFFICACY OF CHLORHEXIDINE AGAINST MUTANS STREPTOCOCCI AND HUMAN DENTAL-CARIES [J].
EMILSON, CG .
JOURNAL OF DENTAL RESEARCH, 1994, 73 (03) :682-691