Nerve growth factor increases sensitivity to bradykinin, mediated through B2 receptors, in capsaicin-sensitive small neurons cultured from rat dorsal root ganglia

被引:27
作者
Kasai, M [1 ]
Kumazawa, T [1 ]
Mizumura, K [1 ]
机构
[1] Nagoya Univ, Environm Med Res Inst, Dept Neural Regulat, Div Regulat Organ Funct,Chikusa Ku, Nagoya, Aichi 4648601, Japan
关键词
nerve growth factor; bradykinin; BK B-1 receptor; BK B-2 receptor; capsaicin; intracellular recording; rat; dorsal root ganglion neuron;
D O I
10.1016/S0168-0102(98)00092-3
中图分类号
Q189 [神经科学];
学科分类号
071006 ;
摘要
To examine the effects of nerve growth factor (NGF) on the response to bradykinin (BK) of primary afferent neurons, intracellular recordings were obtained from small (< 30 mu m) and large (greater than or equal to 35 mu m) neurons in rat dorsal root ganglia (DRG). The response to BK in the small neurons was tested in 23 freshly dissociated neurons (dissociated group), 37 neurons cultured in the absence of NGF (no-NGF group) and 117 neurons in the presence of NGF (NGF group). Application of BK (10(-7) or 10(-5) M) induced a depolarization in a small number of neurons in the freshly dissociated (13%) and the no-NGF (11%) groups. After cultivation with NGF, the percentage of neurons that were depolarized by BK significantly increased to 46% after 2 days of cultivation. In the NGF group, the percentage of neurons sensitive to BK was significantly greater among capsaicin (CAP)-sensitive than among CAP-insensitive neurons (48 vs 20%). This BK-induced depolarization was completely blocked by a B-2 receptor antagonist, but not a B-1 receptor antagonist. With large neurons, in contrast, NGF did not increase the percentage that were BK-sensitive (9% in the dissociated group vs 0% after being cultured 2 days with NGF). These results demonstrate that NGF increases sensitivity to BK, mediated through B-2 receptors only, in capsaicin-sensitive small neurons cultured from rat DRGs. (C) 1998 Elsevier Science Ireland Ltd. All rights reserved.
引用
收藏
页码:231 / 239
页数:9
相关论文
共 49 条
[1]  
AMANN R, 1995, PAIN, V64, P323
[2]   IMMUNOCYTOCHEMICAL LOCALIZATION OF TRKA RECEPTORS IN CHEMICALLY IDENTIFIED SUBGROUPS OF ADULT-RAT SENSORY NEURONS [J].
AVERILL, S ;
MCMAHON, SB ;
CLARY, DO ;
REICHARDT, LF ;
PRIESTLEY, JV .
EUROPEAN JOURNAL OF NEUROSCIENCE, 1995, 7 (07) :1484-1494
[3]  
BEVAN S, 1995, J NEUROSCI, V15, P4918
[4]   UNMYELINATED NOCICEPTORS OF RAT PARASPINAL TISSUES [J].
BOVE, GM ;
LIGHT, AR .
JOURNAL OF NEUROPHYSIOLOGY, 1995, 73 (05) :1752-1762
[5]  
CONKLIN BR, 1988, J PHARMACOL EXP THER, V244, P646
[6]   THE INVOLVEMENT OF BRADYKININ B-1 AND B-2 RECEPTOR MECHANISMS IN CYTOKINE-INDUCED MECHANICAL HYPERALGESIA IN THE RAT [J].
DAVIS, AJ ;
PERKINS, MN .
BRITISH JOURNAL OF PHARMACOLOGY, 1994, 113 (01) :63-68
[7]   ALTERED EXPRESSION OF NERVE GROWTH-FACTOR IN THE SKIN OF TRANSGENIC MICE LEADS TO CHANGES IN RESPONSE TO MECHANICAL STIMULI [J].
DAVIS, BM ;
LEWIN, GR ;
MENDELL, LM ;
JONES, ME ;
ALBERS, KM .
NEUROSCIENCE, 1993, 56 (04) :789-792
[8]   B-1 bradykinin receptors and sensory neurones [J].
Davis, CL ;
Naeem, S ;
Phagoo, SB ;
Campbell, EA ;
Urban, L ;
Burgess, GM .
BRITISH JOURNAL OF PHARMACOLOGY, 1996, 118 (06) :1469-1476
[9]   PULSE EXPOSURE TO PROTEIN-SYNTHESIS INHIBITORS ENHANCES VASCULAR-RESPONSES TO DES-ARG9-BRADYKININ - POSSIBLE ROLE OF INTERLEUKIN-1 [J].
DEBLOIS, D ;
BOUTHILLIER, J ;
MARCEAU, F .
BRITISH JOURNAL OF PHARMACOLOGY, 1991, 103 (01) :1057-1066
[10]   INCREASED CONTENT AND TRANSPORT OF SUBSTANCE-P AND CALCITONIN GENE-RELATED PEPTIDE IN SENSORY NERVES INNERVATING INFLAMED TISSUE - EVIDENCE FOR A REGULATORY FUNCTION OF NERVE GROWTH-FACTOR INVIVO [J].
DONNERER, J ;
SCHULIGOI, R ;
STEIN, C .
NEUROSCIENCE, 1992, 49 (03) :693-698