Evaluation of pyrimidine PNA binding to ssDNA targets from nonequilibrium melting experiments

被引：14

作者：

Lesnik, EA

Risen, LM

Driver, DA

Griffith, MC

Sprankle, K

Freier, SM

机构：

[1] ISIS Pharmaceuticals, Carlsbad, CA 92008

[2] Chiron Viagene, San Diego, CA 92121

[3] Houghten Pharmaceuticals, San Diego, CA 92121

来源：

NUCLEIC ACIDS RESEARCH | 1997年 / 25卷 / 03期

关键词：

D O I：

10.1093/nar/25.3.568

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Slow kinetics of homopyrimidine PNA binding to single stranded DNA and RNA targets is manifested in significant hysteresis in thermal UV absorption experiments, We have compared temperatures of dissociation (T-dis) and reassociation (T-ass) for triplexes formed by DNA and single or bis PNAs with K-50 derived from gel mobility experiments, Results indicated there was no correlation between T-dis and K-50 while reasonable correlation between T-ass and K-50 was found. This correlation enabled use of easy thermal UV absorption experiments for evaluation of PNA binding to DNA/RNA targets.

引用

收藏

页码：568 / 574

页数：7

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