Fabrication and Characterization of Collagen-Immobilized Porous PHBV/HA Nanocomposite Scaffolds for Bone Tissue Engineering

被引：36

作者：

Baek, Jin-Young ^{[1
]}

Xing, Zhi-Cai ^{[1
]}

Kwak, Giseop ^{[1
]}

Yoon, Keun-Byoung ^{[1
]}

Park, Soo-Young ^{[1
]}

Park, Lee Soon ^{[1
]}

Kang, Inn-Kyu ^{[1
]}

机构：

[1] Kyungpook Natl Univ, Dept Polymer Sci & Engn, Taegu 702701, South Korea

来源：

JOURNAL OF NANOMATERIALS | 2012年 / 2012卷

关键词：

SURFACE MODIFICATION; OSTEOBLAST ADHESION; CELL ATTACHMENT; HYDROXYAPATITE; PROLIFERATION; ACID);

D O I：

10.1155/2012/171804

中图分类号：

TB3 [工程材料学];

学科分类号：

0805 ; 080502 ;

摘要：

The porous composite scaffolds (PHBV/HA) consisting of poly (3-hydroxybutyrate-co-3-hydroxyvalerate) (PHBV) and hydroxyapatite (HA) were fabricated using a hot-press machine and salt-leaching. Collagen (type I) was then immobilized on the surface of the porous PHBV/HA composite scaffolds to improve tissue compatibility. The structure and morphology of the collagen-immobilized composite scaffolds (PHBV/HA/Col) were investigated using a scanning electron microscope (SEM), Fourier transform infrared (FTIR), and electron spectroscopy for chemical analysis (ESCA). The potential of the porous PHBV/HA/Col composite scaffolds for use as a bone scaffold was assessed by an experiment with osteoblast cells (MC3T3-E1) in terms of cell adhesion, proliferation, and differentiation. The results showed that the PHBV/HA/Col composite scaffolds possess better cell adhesion and significantly higher proliferation and differentiation than the PHBV/HA composite scaffolds and the PHBV scaffolds. These results suggest that the PHBV/HA/Col composite scaffolds have a high potential for use in the field of bone regeneration and tissue engineering.

引用

页数：11

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