Nitrate stimulation of indigenous nitrate-reducing, sulfide-oxidising bacterial community in wastewater anaerobic biofilms

被引:72
作者
Garcia-de-Lomas, Juan
Corzo, Alfonso
Portillo, M. Carmen
Gonzalez, Juan M.
Andrades, Jose A.
Saiz-Jimenez, Cesareo
Garcia-Robledo, Emilio
机构
[1] Univ Cadiz, Dept Biol, Fac Marine & Environm Sci, Cadiz 11510, Spain
[2] CSIC, IRNAS, Seville 41012, Spain
[3] EMSA, Cadiz 11402, Spain
关键词
wastewater; sulfide; biofilm; nitrate; nitrate-reducing bacteria; MICROBIAL COMMUNITIES; ACTIVATED-SLUDGE; HYDROGEN-SULFIDE; SULFATE; SEQUENCES; OXIDATION; POPULATIONS; INHIBITION; DIVERSITY; SEDIMENTS;
D O I
10.1016/j.watres.2007.04.004
中图分类号
X [环境科学、安全科学];
学科分类号
08 ; 0830 ;
摘要
The role of the nitrate- reducing, sulfide - oxidising bacteria (NR-SOB) in the nitrate-mediated inhibition of sulfide net production by anaerobic wastewater biofilms was analyzed in two experimental bioreactors, continuously fed with the primary effluent of a wastewater treatment plant, one used as control (BRC) and the other one supplemented with nitrate (BRN). This study integrated information from H2S and pH microelectrodes, RNA-based molecular techniques, and the time course of biofilm growth and bioreactors water phase. Biofilms were a net source of sulfide for the water phase (2.01 mu mol S-tot(2-) m(-2) s(-1)) in the absence of nitrate dosing. Nitrate addition effectively led to the cessation of sulfide release from biofilms despite which a low rate of net sulfate reduction activity (0.26 mu mol S-tot(2-) m(-2) s(-1)) persisted at a deep layer within the biofilm. Indigenous NR-SOB including Thiomicrospira denitrificans, Arcobacter sp., and Thiobacillus denitrificans were stimulated by nitrate addition resulting in the elimination of most sulfide from the biofilms. Active sulfate reducing bacteria (SRB) represented comparable fractions of total metabolically active bacteria in the libraries obtained from BRN and BRC. However, we detected changes in the taxonomic composition of the SRB community suggesting its adaptation to a higher level of NR-SOB activity in the presence of nitrate. (C) 2007 Elsevier Ltd. All rights reserved.
引用
收藏
页码:3121 / 3131
页数:11
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