Expression of periostin in the serum of NSCLC and its function on proliferation and migration of human lung adenocarcinoma cell line (A549) in vitro

被引:67
作者
Hong, Lingzhi [1 ]
Sun, Huiming [1 ]
Lv, Xiaojing [1 ]
Yang, Di [2 ]
Zhang, Jinan [2 ]
Shi, Yi [1 ]
机构
[1] Nanjing Univ, Jinling Hosp, Sch Med, Dept Resp Dis, Nanjing 210002, Jiangsu Prov, Peoples R China
[2] Nanjing Med Univ, Affiliated Hosp 1, Inst Cardiol, Nanjing 210029, Jiangsu Prov, Peoples R China
关键词
NSCLC; Periostin; A549; Proliferation; Migration; EMT; CANCER CELLS; ADHESION; GROWTH; INVASION; OSTEOBLAST; PROTEIN; METASTASIS; TRANSITION; INDUCTION; CARCINOMA;
D O I
10.1007/s11033-009-9721-1
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Periostin is over expressed in many epithelial malignant cancers, including lung cancer, breast cancer, ovarian cancer and colon cancer. It is related with the progression and migration of breast and ovarian cancer cells in vitro. The aim of this study was to investigate the serum level of periostin in non-small cell lung cancer (NSCLC) and its relationship with established biological and prognostic factors by enzyme-linked-immunosorbent serologic assay. We also observe the function of periostin on the proliferation and migration of human lung adenocarcinoma cell line (A549) and discuss the mechanism. The mean value for serum periostin (POSTN) was elevated in NSCLC patients (242.84 +/- A 5.33 pg/ml) compared to the normal healthy volunteers (215.66 +/- A 11.67 pg/ml) (p = 0.030). The serum level of periostin of NSCLC patients had no connection with gender, age, pathological type, TNM stage, lymph node status, tumor size and invasiveness. We constructed a plasmid named pEGFP-N1/POSTN expressing full-length human periostin. Transfecting the plasmid to A549 cells and periostin was efficiently expressed in transfected A549 cells. Our data showed that periostin could promote the proliferation and migration of A549 cells by inducing vimentin and N-cadherin expression and downregulating E-cadherin expression. These results strongly suggest that periostin is a novel molecular which play an important role during the progression and development of NSCLC.
引用
收藏
页码:2285 / 2293
页数:9
相关论文
共 40 条
[1]   Periostin potently promotes metastatic growth of colon cancer by augmenting cell survival via the Akt/PKB pathway [J].
Bao, SD ;
Ouyang, G ;
Bai, XF ;
Huang, Z ;
Ma, CY ;
Liu, M ;
Shoo, R ;
Anderson, RM ;
Rich, JN ;
Wang, XF .
CANCER CELL, 2004, 5 (04) :329-339
[2]   Periostin promotes invasiveness and resistance of pancreatic cancer cells to hypoxia-induced cell death:: role of the β4 integrin and the PI3k pathway [J].
Baril, P. ;
Gangeswaran, R. ;
Mahon, P. C. ;
Caulee, K. ;
Kocher, H. M. ;
Harada, T. ;
Zhu, M. ;
Kalthoff, H. ;
Crnogorac-Jurcevic, T. ;
Lemoine, N. R. .
ONCOGENE, 2007, 26 (14) :2082-2094
[3]   Altered expression of adhesion molecules and epithelial-mesenchymal transition in silica-induced rat lung carcinogenesis [J].
Blanco, D ;
Vicent, S ;
Elizegi, E ;
Pino, I ;
Fraga, MF ;
Esteller, M ;
Saffiotti, U ;
Lecanda, F ;
Montuenga, LM .
LABORATORY INVESTIGATION, 2004, 84 (08) :999-1012
[4]   The E-cadherin cell-cell adhesion complex and lung cancer invasion, metastasis, and prognosis [J].
Bremnes, RM ;
Veve, R ;
Hirsch, FR ;
Franklin, WA .
LUNG CANCER, 2002, 36 (02) :115-124
[5]   Cell adhesion and signalling by cadherins and Ig-CAMs in cancer [J].
Cavallaro, U ;
Christofori, G .
NATURE REVIEWS CANCER, 2004, 4 (02) :118-132
[6]   Differential expression of osteoblast-specific factor 2 and polymeric immunoglobulin receptor genes in nasopharyngeal carcinoma [J].
Chang, Y ;
Lee, TC ;
Li, JC ;
Lai, TL ;
Chua, HH ;
Chen, CL ;
Doong, SL ;
Chou, CK ;
Sheen, TS ;
Tsai, CH .
HEAD AND NECK-JOURNAL FOR THE SCIENCES AND SPECIALTIES OF THE HEAD AND NECK, 2005, 27 (10) :873-882
[7]   Gaseous Nitrogen Oxide Promotes Human Lung Cancer Cell Line A549 Migration, Invasion, and Metastasis via iNOS-Mediated MMP-2 Production [J].
Chen, Jing-Hsien ;
Lin, Hui-Hsuan ;
Chiang, Tai-An ;
Hsu, Jeng-Dong ;
Ho, Hsieh-Hsun ;
Lee, Yi-Chieh ;
Wang, Chau-Jong .
TOXICOLOGICAL SCIENCES, 2008, 106 (02) :364-375
[8]   CAM-FGF receptor interactions: A model for axonal growth [J].
Doherty, P ;
Walsh, FS .
MOLECULAR AND CELLULAR NEUROSCIENCE, 1996, 8 (2-3) :99-111
[9]  
Gillan L, 2002, CANCER RES, V62, P5358
[10]   EPITHELIA SUSPENDED IN COLLAGEN GELS CAN LOSE POLARITY AND EXPRESS CHARACTERISTICS OF MIGRATING MESENCHYMAL CELLS [J].
GREENBURG, G ;
HAY, ED .
JOURNAL OF CELL BIOLOGY, 1982, 95 (01) :333-339