Comparison of the TaqMan and LightCycler systems in evaluation of CYP2B6 516G>T polymorphism

被引：4

作者：

Chantarangsu, Soranun

Cressey, Tim

Mahasirimongkol, Surakameth

Tawon, Yardpiroon

Ngo-Giang-Huong, Nicole

Jourdain, Gonzague

Lallemant, Marc

Chantratita, Wasun

机构：

[1] Mahidol Univ, Ramathibodi Hosp, Fac Med, Dept Pathol, Bangkok 10400, Thailand

[2] Chiang Mai Univ, PHPT, Chiang Mai 50000, Thailand

[3] Minist Publ Hlth, Ctr Int Cooperat, Dept Med Sci, Nonthaburi, Thailand

来源：

MOLECULAR AND CELLULAR PROBES | 2007年 / 21卷 / 5-6期

关键词：

cytochrome P450 (CYP450); pharmacogenetic testing; SimpleProbe probe; TaqMan probe;

D O I：

10.1016/j.mcp.2007.06.008

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Understanding genetically encoded inherited differences in drug metabolism and drug targets offers the promise of minimizing adverse drug reactions and improving therapies. We have compared two real-time PCR-based methods, the TaqMan((R)) and LightCycler (TM) for the pharmacogenctic evaluation of CYP2B6 516G > T polymorphism. Both methods were found to be suitable for pharmacogenetic testing of CYP2B6 516G > T in the meaning of time consumption, accurate genotype calling, flexible reaction format, simple data analysis, and low cost per assay. The genotype success rate was similar, but the LightCycler procedure was less expensive in terms of cost per sample and shorter running time. (c) 2007 Elsevier Ltd. All rights reserved.

引用

页码：408 / 411

页数：4

共 18 条

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