High-resolution functional proteomics by active-site peptide profiling

被引：77

作者：

Okerberg, ES ^{[1
]}

Wu, JY ^{[1
]}

Zhang, BH ^{[1
]}

Samii, B ^{[1
]}

Blackford, K ^{[1
]}

Winn, DT ^{[1
]}

Shreder, KR ^{[1
]}

Burbaum, JJ ^{[1
]}

Patricelli, MP ^{[1
]}

机构：

[1] ActivX Biosci, La Jolla, CA 92037 USA

来源：

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA | 2005年 / 102卷 / 14期

关键词：

capillary electrophoresis; fluorescence; MS; protease;

D O I：

10.1073/pnas.0501205102

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

Characterization and functional annotation of the large number of proteins predicted from genome sequencing projects poses a major scientific challenge. Whereas several proteomics techniques have been developed to quantify the abundance of proteins, these methods provide little information regarding protein function. Here, we present a gel-free platform that permits ultrasensitive, quantitative, and high-resolution analyses of protein activities in proteomes, including highly problematic samples such as undiluted plasma. We demonstrate the value of this platform for the discovery of both disease-related enzyme activities and specific inhibitors that target these proteins.

引用

页码：4996 / 5001

页数：6

共 24 条

[1] Chemical strategies for functional proteomics [J].