Surfactant Enhance DLLME/FO-LADS: Assay of Malachite Green Level in Aquatic Environment of Trout Fish

Malachite green (MG), a traditional agent used in aquaculture although is not approved; its low cost and high efficacy make illicit use likely. We developed a small-scale, simple, and sensitive dispersive liquid-liquid microextraction procedure for the assay of trace amounts of MG in aquatic environment of Trout fish. Fiber optic-linear array detection spectrophotometry with charge-coupled device detector benefiting from a microcell was used for this purpose. The method is based on enhancement effect of an anionic surfactant on the extraction of MG in to very fine multidroplets of microextraction solvent which made assisted by disperser solvent. Under the optimum conditions, the enrichment factor 77.5 was obtained from a 5-mL water sample. The calibration graph was linear up to 5 x 10(-7) mol L-1 with detection limit of 1 x 10(-8) mol L-1. The relative standard deviation for seven replicate measurements of 4 x 10(-7) and 5 x 10(-8) mol L-1 of MG were 3.3 and 4.5%, respectively.