Construction of an oilseed rape (Brassica napus L.) genetic map with SSR markers

被引：243

作者：

Piquemal, J ^{[1
]}

Cinquin, E ^{[1
]}

Couton, F ^{[1
]}

Rondeau, C ^{[1
]}

Seignoret, E ^{[1
]}

Doucet, I ^{[1
]}

Perret, D ^{[1
]}

Villeger, MJ ^{[1
]}

Vincourt, P ^{[1
]}

Blanchard, P ^{[1
]}

机构：

[1] Euralis Semences Lab Genet Mol, F-31700 Mondonville, France

来源：

THEORETICAL AND APPLIED GENETICS | 2005年 / 111卷 / 08期

关键词：

D O I：

10.1007/s00122-005-0080-6

中图分类号：

S3 [农学（农艺学）];

学科分类号：

0901 ;

摘要：

We constructed a Brassica napus genetic map with 240 simple sequence repeats (SSR) primer pairs from private and public origins. SSR, or microsatellites, are highly polymorphic and efficient markers for the analysis of plant genomes. Our selection of primer pairs corresponded to 305 genetic loci that we were able to map. In addition, we also used 52 sequence-characterized amplified region primer pairs corresponding to 58 loci that were developed in our lab. Genotyping was performed on six F2 populations, corresponding to a total of 574 F2 individual plants, obtained according to an unbalanced diallel cross design involving six parental lines. The resulting consensus map presented 19 linkage groups ranging from 46.2 to 276.5 cM, which we were able to name after the B. napus map available at http://ukcrop.net/perl/ace/search/BrassicaDB, thus enabling the identification of the A genome linkage groups originating from the B. rapa ancestor and the C genome linkage groups originating from the B. oleracea ancestor in the amphidiploid genome of B. napus. Some homoeologous regions were identified between the A and the C genomes. This map could be used to identify more markers, which would eventually be linked to genes controlling important agronomic characters in rapeseed. Furthermore, considering the good genome coverage we obtained, together with an observed homogenous distribution of the loci across the genome, this map is a powerful tool to be used in marker-assisted breeding.

引用

页码：1514 / 1523

页数：10

共 21 条

[1]

BUDIN K, 2004, PLANT AN GEN 12 C JA

[2] Comparison of the genetic maps of Brassica napus and Brassica oleracea [J].

Cheung, WY ;

Champagne, G ;

Hubert, N ;

Landry, BS .

THEORETICAL AND APPLIED GENETICS, 1997, 94 (05) :569-582

[3]

Dellaporta S. L., 1983, Plant Molecular Biology Reporter, V1, P19, DOI [10.1007/BF02712670, DOI 10.1007/BF02712670]

[4] RFLP MAPPING OF BRASSICA-NAPUS USING DOUBLED HAPLOID LINES [J].

FERREIRA, ME ;

WILLIAMS, PH ;

OSBORN, TC .

THEORETICAL AND APPLIED GENETICS, 1994, 89 (05) :615-621

[5]

Foisset N, 1996, THEOR APPL GENET, V93, P1017, DOI 10.1007/BF00230119

[6]

Foisset N, 1996, IN VITRO HAPLOID PRO, P189

[7] A GENETIC-MAP FOR BRASSICA-NAPUS BASED ON RESTRICTION-FRAGMENT-LENGTH-POLYMORPHISMS DETECTED WITH EXPRESSED DNA-SEQUENCES [J].

LANDRY, BS ;

HUBERT, N ;

ETOH, T ;

HARADA, JJ ;

LINCOLN, SE .

GENOME, 1991, 34 (04) :543-552

[8] A consensus linkage map for rapeseed (Brassica napus L.):: construction and integration of three individual maps from DH populations [J].

Lombard, V ;

Delourme, R .

THEORETICAL AND APPLIED GENETICS, 2001, 103 (04) :491-507

[9] Efficient large-scale development of microsatellites for marker and mapping applications in Brassica crop species [J].

Lowe, AJ ;

Moule, C ;

Trick, M ;

Edwards, KJ .

THEORETICAL AND APPLIED GENETICS, 2004, 108 (06) :1103-1112

[10]

Osborn TC, 1997, GENETICS, V146, P1123

← 1 2 3 →