A human homolog of mouse Lbh gene, hLBH, expresses in heart and activates SRE and AP-1 mediated MAPK signaling pathway

被引：33

作者：

Ai, Jianping ^{[1
]}

Wang, Yuequn ^{[1
]}

Tan, Kunrong ^{[1
]}

Deng, Yun ^{[1
]}

Luo, Na ^{[1
]}

Yuan, Wuzhou ^{[1
]}

Wang, Zequn ^{[1
]}

Li, Yongqing ^{[1
]}

Wang, Ying ^{[1
]}

Mo, Xiaoyang ^{[1
]}

Zhu, Chuanbing ^{[1
]}

Yin, Zhaochu ^{[1
]}

Liu, Mingyao ^{[1
]}

Wu, Xiushan ^{[1
]}

机构：

[1] Hunan Normal Univ, Coll Life Sci, Ctr Heart Dev, Key Lab MOE Dev Biol & Protein Chem, Changsha 410081, Hunan, Peoples R China

来源：

MOLECULAR BIOLOGY REPORTS | 2008年 / 35卷 / 02期

基金：

中国国家自然科学基金;

关键词：

hLBH; transcription factor; gene regulation; SRE; AP-1;

D O I：

10.1007/s11033-007-9068-4

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

It has been reported that mouse Lbh (limb-bud and heart) can regulate cardiac gene expression by modulating the combinatorial activities of key cardiac transcription factors, as well as their individual functions in cardiogenesis. Here we report the cloning and characterization of the human homolog of mouse Lbh gene, hLBH, from a human embryonic heart cDNA library. The cDNA of hLBH is 2927 bp long, encoding a protein product of 105 amino acids. The protein is highly conserved in evolution across different species from zebra fish, to mouse, to human. Northern blot analysis indicates that a 2.9 kb transcript specific for hLBH is most abundantly expressed in both embryonic and adult heart tissue. In COS-7 cells, hLBH proteins are localized to both the nucleus and the cytoplasm. hLBH is a transcription activator when fused to Gal-4 DNA-binding domain. Deletion analysis indicates that both the N-terminal containing proline-dependent serine/threonine kinase group and the C-terminal containing ERK D-domain motif are required for transcriptional activation. Overexpression of hLBH in COS-7 cells activates the transcriptional activities of activator protein-1 (AP-1) and serum response element (SRE). These results suggest that hLBH proteins may act as a transcriptional activator in mitogen-activated protein kinase signaling pathway to mediate cellular functions.

引用

页码：179 / 187

页数：9

共 22 条

[1]

Aggeli IKS, 2002, J EXP BIOL, V205, P443

[2] Congenital heart disease reminiscent of partial trisomy 2p syndrome in mice transgenic for the transcription factor Lbh [J].