A new luminescence assay for autoantibodies to mammalian cell-prepared insulinoma-associated protein 2

被引：29

作者：

Burbelo, Peter D. ^{[1
]}

Hirai, Hiroki ^{[2
]}

Leahy, Hannah ^{[1
]}

Lernmark, Ake ^{[3
]}

Ivarsson, Sten A. ^{[4
]}

Iadarola, Michael J. ^{[1
]}

Notkins, Abner Louis ^{[2
]}

机构：

[1] Natl Inst Dent & Craniolacial Res, Sensory Biol Branch, Natl Inst Hlth, Bethesda, MD USA

[2] Natl Inst Dental & Craniofacial Res, Oral Infect & Immun Branch, Natl Inst Hlth, Bethesda, MD USA

[3] Univ Washington, Dept Clin Sci, RH Williams Lab, Seattle, WA 98195 USA

[4] Lund Univ, Univ Hosp MAS, Dept Clin Sci, Malmo, Sweden

来源：

DIABETES CARE | 2008年 / 31卷 / 09期

基金：

美国国家卫生研究院;

关键词：

D O I：

10.2337/dc08-0286

中图分类号：

R5 [内科学];

学科分类号：

1002 ; 100201 ;

摘要：

OBJECTIVE - Insulinoma-associated protein 2 (IA-2) is a major autoantigen in type I diabetes, and IA-2 autoantibodies are routinely detected by a liquid-phase radioimmunoprecipitation assay. The present experiments were initiated to develop a new assay that does not require the use of radioisotopes or autoantigens prepared in bacteria or by in vitro transcription/translation. RESEARCH DESIGN AND METHODS - IA-2 luciferase fusion protein was expressed in mammalian cells and assayed for autoantibodies by liquid-phase luciferase immunoprecipitation. RESULTS - Our study showed that there was no significant difference between the luciferase immunoprecipitation and the radioimmunoprecipitation assays in sensitivity and specificity, and comparison of the two assays revealed a high correlation coefficient (R-2 = 0.805). CONCLUSIONS - The luciferase system offers a robust, inexpensive, nonradioactive method for the detection of autoantibodies to mammalian cell-prepared IA-2 and could be of practical value at the clinical level.

引用

页码：1824 / 1826

页数：3

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