Dye coupling in Purkinje cells of organotypic slice cultures

被引:16
作者
Meller, K [1 ]
Krah, K [1 ]
Theiss, C [1 ]
机构
[1] Ruhr Univ Bochum, Abt Cytol, Fak Med, Inst Anat, D-44780 Bochum, Germany
来源
DEVELOPMENTAL BRAIN RESEARCH | 2005年 / 160卷 / 01期
关键词
cerebellar slice culture; dye spreading; gap junction; microinjection; Purkinje cell;
D O I
10.1016/j.devbrainres.2005.08.007
中图分类号
Q [生物科学];
学科分类号
07 ; 0710 ; 09 ;
摘要
Cerebellar slice cultures of newborn rats showed poorly developed dendritic arborization of Purkinje cells, whereas cultures of 10-day-old rats revealed prominent dendritic branching. Gap junctional intercellular communication between Purkinje cells, investigated as dye transfer of microinjected neurobiotin, occurred through dendro-dendritic contacts, with decreased dye spreading in old cell cultures. These results indicate a possible correlation of gap junctional intercellular communication and the development of Purkinje cells. (c) 2005 Elsevier B.V. All rights reserved.
引用
收藏
页码:101 / 105
页数:5
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