Lysozyme net charge and ion binding in concentrated aqueous electrolyte solutions

被引:235
作者
Kuehner, DE
Engmann, J
Fergg, F
Wernick, M
Blanch, HW
Prausnitz, JM [1 ]
机构
[1] Univ Calif Berkeley, Dept Chem Engn, Berkeley, CA 94720 USA
[2] Lawrence Berkeley Lab, Div Chem Sci, Berkeley, CA 94720 USA
来源
JOURNAL OF PHYSICAL CHEMISTRY B | 1999年 / 103卷 / 08期
关键词
D O I
10.1021/jp983852i
中图分类号
O64 [物理化学(理论化学)、化学物理学];
学科分类号
070304 ; 081704 ;
摘要
Hydrogen-ion titrations were conducted for hen-egg-white lysozyme in solutions of potassium chloride over the range pH 2.5-11.5 and for ionic strengths to 2.0 M. The dependence of lysozyme's net proton charge, z(P), on pH and ionic strength in potassium chloride solution is measured. From the ionic-strength dependence of z(P), interactions of lysozyme with potassium and chloride ions are calculated using the molecular-thermodynamic theory of Fraaije and Lyklema.(1) Lysozyme interacts preferentially with up to 12 chloride ions at pH 2.5. The observed dependence of ion-protein interactions on pH and ionic strength is explained in terms of electric-double-layer theory. New experimental pK(a) data are reported for 11 amino acids in potassium chloride solutions of ionic strength to 3.0 M.
引用
收藏
页码:1368 / 1374
页数:7
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