Protein adsorption, fibroblast activity and antibacterial properties of poly(3-hydroxybutyric acid-co-3-hydroxyvaleric acid) grafted with chitosan and chitooligosaccharide after immobilized with hyaluronic acid

被引：122

作者：

Hu, SG ^{[1
]}

Jou, CH ^{[1
]}

Yang, MC ^{[1
]}

机构：

[1] Natl Taiwan Univ Sci & Technol, Dept Polymer Engn, Taipei 10672, Taiwan

来源：

BIOMATERIALS | 2003年 / 24卷 / 16期

关键词：

poly(3-hydroxybutyric acid-co-3-hydroxyvaleric acid; chitosan; antibacterial activity; protein adhesion; hyaluronic acid;

D O I：

10.1016/S0142-9612(03)00079-6

中图分类号：

R318 [生物医学工程];

学科分类号：

0831 ;

摘要：

Poly(3-hydroxybutyric acid-co-3-hydroxyvaleric acid) (PHBV) membrane was treated with ozone and grafted with acrylic acid. The resulting membranes were further grafted with chitosan (CS) or chitooligosaccharide (COS) via esterification. Afterward hyaluronic acid (HA) was immobilized onto CS- or COS-grafting membranes. The antibacterial activity of CS and COS against Staphylococus aureus, Escherichia coli, and Pseudomonas aeruginosa was preserved after HA immobilization. Among them, CS-grafted PHBV membrane. In addition, after CS- or COS-grafted PHBV membrane showed higher antibacterial activity than COS grafting, the L929 fibroblasts attachment and protein adsorption were improved, while the cell number was decrease. After immobilizing HA, the cell proliferation was promoted, the protein adsorption was decreased, and the cell attachment was slightly lower than CS- or COS-grafting PHBV. (C) 2003 Elsevier Science Ltd. All rights reserved.

引用

页码：2685 / 2693

页数：9

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