Separation of lac dye components by high-speed counter-current chromatography

被引：46

作者：

Oka, H

Ito, Y

Yamada, S

Kagami, T

Hayakawa, J

Harada, K

Atsumi, E

Suzuki, M

Odani, H

Akahori, S

Maeda, K

Nakazawa, H

Ito, Y

机构：

[1] Aichi Prefectural Inst Publ Hlth, Kita Ku, Nagoya, Aichi 462, Japan

[2] Dept Hlth, Food Safety & Vet Affairs Div, Naka Ku, Nagoya, Aichi 46001, Japan

[3] Meijo Univ, Fac Pharm, Tempaku Ku, Nagoya, Aichi 468, Japan

[4] Nagoya Univ, Branch Hosp, Dept Internal Med, Nagoya, Aichi 461, Japan

[5] Hoshi Univ, Fac Pharmaceut Sci, Shinagawa Ku, Tokyo 142, Japan

[6] NHLBI, Biophys Chem Lab, NIH, Bethesda, MD 20892 USA

来源：

JOURNAL OF CHROMATOGRAPHY A | 1998年 / 813卷 / 01期

关键词：

counter-current chromatography; food analysis; laccaic acids; lac dye; dyes;

D O I：

10.1016/S0021-9673(98)00311-2

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

High-speed counter-current chromatography has been successfully applied to the separation of the lac dye components. A 25-mg quantity of the sample was separated using a two-phase solvent system composed of tert.-butyl methyl ether-n-butanol-acetonitrile-water (2.2:1:5). The fractions were analyzed by high-performance liquid chromatography and electrospray tandem mass spectrometry. The separation yielded 2.6 mg of 97.2% purl laccaic acid C, 9.5 mg of 98.1% pure laccaic acid A, 3.6 mg of 98.2% pure laccaic acid B, and 0.5 mg of a 95.0% pure anthraquinonedicarboxylic acid with a molecular mass of 360. (C) 1998 Elsevier Science B.V. All rights reserved.

引用

页码：71 / 77

页数：7

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