Short-term oleoyl-estrone treatment affects capacity to manage lipids in rat adipose tissue

被引:19
作者
Salas, Anna
Noe, Veronique
Ciudad, Carlos J.
Romero, M. Mar
Remesar, Xavier
Esteve, Montserrat
机构
[1] Univ Barcelona, Fac Biol, Dept Nutr & Bromatol, E-08028 Barcelona, Spain
[2] Univ Barcelona, Fac Farm, Dept Bioquim & Biol Mol, E-08028 Barcelona, Spain
关键词
D O I
10.1186/1471-2164-8-292
中图分类号
Q81 [生物工程学(生物技术)]; Q93 [微生物学];
学科分类号
071005 ; 0836 ; 090102 ; 100705 ;
摘要
Background: Short-term OE (oleoyl-estrone) treatment causes significant decreases in rat weight mainly due to adipose tissue loss. The aim of this work was to determine if OE treatment affects the expression of genes that regulate lipid metabolism in white adipose tissue. Results: Gene expression in adipose tissue from female treated rats (48 hours) was analysed by hybridization to cDNA arrays and levels of specific mRNAs were determined by real-time PCR. Treatment with OE decreased the expression of 232 genes and up-regulated 75 other genes in mesenteric white adipose tissue. The use of real-time PCR validate that, in mesenteric white adipose tissue, mRNA levels for Lipoprotein Lipase (LPL) were decreased by 52%, those of Fatty Acid Synthase (FAS) by 95%, those of Hormone Sensible Lipase (HSL) by 32%, those of Acetyl CoA Carboxylase (ACC) by 92%, those of Carnitine Palmitoyltransferase 1b (CPT1b) by 45%, and those of Fatty Acid Transport Protein 1 (FATP1) and Adipocyte Fatty Acid Binding Protein (FABP4) by 52% and 49%, respectively. Conversely, Tumour Necrosis Factor (TNF alpha) values showed overexpression (198%). Conclusion: Short-term treatment with OE affects adipose tissue capacity to extract fatty acids from lipoproteins and to deal with fatty acid transport and metabolism.
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页数:8
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共 29 条
[1]   Weight loss in a patient with morbid obesity under treatment with oleoyl-estrone [J].
Alemany, M ;
Fernández-López, JA ;
Petrobelli, A ;
Granada, M ;
Foz, M ;
Remesar, X .
MEDICINA CLINICA, 2003, 121 (13) :496-499
[2]  
BOWER JF, 2005, AM J PHYSIOL, V290, pE87
[3]   Short-term oral oleoyl-estrone treatment increases plasma cholesterol turnover in the rat [J].
Cabot, C ;
Salas, A ;
Ferrer-Lorente, R ;
Savall, P ;
Remesar, X ;
Fernández-López, JA ;
Esteve, M ;
Alemany, M .
INTERNATIONAL JOURNAL OF OBESITY, 2005, 29 (05) :534-539
[4]   Oleoyl-estrone does not have direct estrogenic effects on rats [J].
Cabot, C ;
Grasa, MDM ;
Massanés, RM ;
de Matteis, R ;
Cinti, S ;
Fernández-López, JA ;
Remesar, X ;
Alemany, M .
LIFE SCIENCES, 2001, 69 (07) :749-761
[5]   Plasma acyl-estrone levels are altered in obese women [J].
Cabot, C ;
Masanés, R ;
Bullo, M ;
García-Lorda, P ;
Fernández-López, JA ;
Salas-Salvadó, J ;
Alemany, M .
ENDOCRINE RESEARCH, 2000, 26 (03) :465-476
[6]   Coordinated upregulation of oxidative pathways and downregulation of lipid biosynthesis underlie obesity resistance in perilipin knockout mice - A microarray gene expression profile [J].
Castro-Chavez, F ;
Yechoor, VK ;
Saha, PK ;
Martinez-Botas, J ;
Wooten, EC ;
Sharma, S ;
O'Connell, P ;
Taegtmeyer, H ;
Chan, L .
DIABETES, 2003, 52 (11) :2666-2674
[7]   Pro-inflammatory cytokines and adipose tissue [J].
Coppack, SW .
PROCEEDINGS OF THE NUTRITION SOCIETY, 2001, 60 (03) :349-356
[8]   Genetic variation in fatty acid-binding protein-4 and peroxisome proliferator-activated receptor γ interactively influence insulin sensitivity and body composition in males [J].
Damcott, CM ;
Moffett, SP ;
Feingold, E ;
Barmada, MM ;
Marshall, JA ;
Hamman, RF ;
Ferrell, RE .
METABOLISM-CLINICAL AND EXPERIMENTAL, 2004, 53 (03) :303-309
[9]   Expression of a cDNA isolated from rat brown adipose tissue and heart identifies the product as the muscle isoform of carnitine palmitoyltransferase I (M-CPT I) - M-CPT I is the predominant CPT I isoform expressed in both white (epididymal) and brown adipocytes [J].
Esser, V ;
Brown, NF ;
Cowan, AT ;
Foster, DW ;
McGarry, JD .
JOURNAL OF BIOLOGICAL CHEMISTRY, 1996, 271 (12) :6972-6977
[10]   Modulation by leptin, insulin and corticosterone of oleoyl-estrone synthesis in cultured 3T3 L1 cells [J].
Esteve, M ;
Savall, P ;
Virgilli, J ;
Fernández-López, JA ;
Remesar, X ;
Alemany, M .
BIOSCIENCE REPORTS, 2001, 21 (06) :755-763