Method for trapping and manipulating nanoscale objects in solution

被引:192
作者
Cohen, AE [1 ]
Moerner, WE
机构
[1] Stanford Univ, Dept Phys, Stanford, CA 94305 USA
[2] Stanford Univ, Dept Chem, Stanford, CA 94305 USA
关键词
D O I
10.1063/1.1872220
中图分类号
O59 [应用物理学];
学科分类号
摘要
We present a device that allows a user to trap a single nanoscale object in solution at ambient temperature, and then to position the trapped object with nanoscale resolution. This anti-Brownian electrophoretic trap (ABEL trap) works by monitoring the Brownian motion of the particle (via fluorescence microscopy), and then applying a feedback voltage to the solution so that the electrophoretic drift exactly cancels the Brownian motion. The ABEL trap works on any object that can be imaged optically and that acquires a charge in water. The ABEL trap is noninvasive, is gentle enough to handle biological molecules, and can trap objects far smaller than can be trapped with laser tweezers. Our proof-of-principle device can trap fluorescent polystyrene nanospheres with diameters down to 20 nm. (C) 2005 American Institute of Physics.
引用
收藏
页码:1 / 3
页数:3
相关论文
共 7 条
[1]   OBSERVATION OF A SINGLE-BEAM GRADIENT FORCE OPTICAL TRAP FOR DIELECTRIC PARTICLES [J].
ASHKIN, A ;
DZIEDZIC, JM ;
BJORKHOLM, JE ;
CHU, S .
OPTICS LETTERS, 1986, 11 (05) :288-290
[2]   Tracking of fluorescent molecules diffusing within membranes [J].
Enderlein, J .
APPLIED PHYSICS B-LASERS AND OPTICS, 2000, 71 (05) :773-777
[3]   Dielectrophoresis-based sample handling in general-purpose programmable diagnostic instruments [J].
Gascoyne, PRC ;
Vykoukal, JV .
PROCEEDINGS OF THE IEEE, 2004, 92 (01) :22-42
[4]   Magnetic tweezers: Micromanipulation and force measurement at the molecular level [J].
Gosse, C ;
Croquette, V .
BIOPHYSICAL JOURNAL, 2002, 82 (06) :3314-3329
[5]  
Jones T.B., 2005, Electromechanics of particles
[6]   A dozen years of single-molecule spectroscopy in physics, chemistry, and biophysics [J].
Moerner, WE .
JOURNAL OF PHYSICAL CHEMISTRY B, 2002, 106 (05) :910-927
[7]   Holding forces of single-particle dielectrophoretic traps [J].
Voldman, J ;
Braff, RA ;
Toner, M ;
Gray, ML ;
Schmidt, MA .
BIOPHYSICAL JOURNAL, 2001, 80 (01) :531-541