Identification and distribution of tryptophan hydroxylase (TPH)-positive neurons in the planarian Dugesia japonica

被引：78

作者：

Nishimura, Kaneyasu

Kitamura, Yoshihisa ^{[1
]}

Inoue, Takeshi

Umesono, Yoshihiko

Yoshimoto, Kanji

Takeuchi, Kosei

Taniguchi, Takashi

Agata, Kiyokazu

机构：

[1] Kyoto Univ, Grad Sch Sci, Dept Biophys, Sakyo Ku, Kyoto 606, Japan

[2] Kyoto Pharmaceut Univ, 21st Century COE Program, Dept Neurobiol, Kyoto 607, Japan

[3] RIKEN, Ctr Dev Biol, Grp Evolut Reg Biol, Kobe, Hyogo 650, Japan

[4] Kobe MI R&D Ctr, KAN Res Inst Inc, Kobe, Hyogo 650, Japan

[5] Kyoto Univ, Grad Sch Sci, Dept Biophys, Kyoto 606, Japan

[6] Kyoto Prefectural Univ Med, Dept Legal Med, Kyoto 602, Japan

来源：

NEUROSCIENCE RESEARCH | 2007年 / 59卷 / 01期

关键词：

planarian; tryptophan hydroxylase; serotonergic neurons; neural network; HPLC; RNA interference;

D O I：

10.1016/j.neures.2007.05.014

中图分类号：

Q189 [神经科学];

学科分类号：

071006 ;

摘要：

We identified a full-length tryptophan hydroxylase (TPH) gene of planarian Dugesia japonica from a head EST database, and named it DjTPH. Based on whole-mount in situ hybridization and immunofluorescence analyses, DjTPH mRNA and protein were mainly expressed in the nervous system, especially ventral nerve cords and eye pigment cells. Furthermore, DjTPH immunoreactivity was clearly detected at commissure axonal connections in the ventral nerve cords. 5-HT was significantly decreased in DjTPH-knockdown planarians compared with control animals. These results suggest that DjTPH is required for 5-HT biosynthesis, and DjTPH antibody is a useful marker for serotonergic neurons in planarians. (C) 2007 Elsevier Ireland Ltd and the Japan Neuroscience Society. All rights reserved.

引用

页码：101 / 106

页数：6

共 21 条

[1] Structure of the planarian central nervous system (CNS) revealed by neuronal cell markers [J].