Facile DNA Immobilization on Surfaces through a Catecholamine Polymer

被引:230
作者
Ham, Hyun Ok [1 ]
Liu, Zhongqiang [1 ]
Lau, K. H. Aaron [1 ]
Lee, Haeshin [2 ,3 ]
Messersmith, Phillip B. [1 ,4 ,5 ]
机构
[1] Northwestern Univ, Dept Biomed Engn, Evanston, IL 60208 USA
[2] Korea Adv Inst Sci & Technol, Dept Chem, Taejon 305701, South Korea
[3] Korea Adv Inst Sci & Technol, Grad Sch Nanosci & Technol WCU, Taejon 305701, South Korea
[4] Northwestern Univ, Dept Mat Sci & Engn, Chem & Biol Engn Dept, Chem Life Proc Inst,Inst Bionanotechnol Med, Evanston, IL 60208 USA
[5] Northwestern Univ, Robert H Lurie Comprehens Canc Ctr, Evanston, IL 60208 USA
基金
新加坡国家研究基金会;
关键词
biomimetic synthesis; biosensors; catecholamine; DNA immobilization; surface modification; COVALENT ATTACHMENT; TOF-SIMS; MICROARRAY; OLIGONUCLEOTIDES; FLUORESCENCE; STRATEGIES; GOLD; XPS; IDENTIFICATION; FABRICATION;
D O I
10.1002/anie.201005001
中图分类号
O6 [化学];
学科分类号
070301 [无机化学];
摘要
Simple immersion of noble metals, oxides, semiconductors, and synthetic polymer substrates in a mussel-mimetic catecholamine polymer solution p(DOMA-AEMA) leads to formation of a thin film on the substrate. The resulting coated substrates can bind DNA without further surface treatment. This approach provides a new entrance to DNA microarray fabrication. Copyright © 2011 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.
引用
收藏
页码:732 / 736
页数:5
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