RhoA regulates lipopolysaccharide-induced lung cell injury via the Wnt/β-catenin pathway

被引：8

作者：

Chen, Guanhua ^{[1
]}

Sun, Xuedong ^{[1
]}

Dong, Chunxiao ^{[2
]}

机构：

[1] Cent Hosp Shengli Oil Field Shandong, Dept Emergency, 31 Jinan Rd, Dongying 257000, Shandong, Peoples R China

[2] Cent Hosp Shengli Oil Field Shandong, Dept Pediat, Dongying 257000, Shandong, Peoples R China

来源：

MOLECULAR MEDICINE REPORTS | 2017年 / 16卷 / 06期

关键词：

acute lung injury; lipopolysaccharide; Ras homolog family member A; Wnt/beta-catenin; MICE; REGENERATION; FIBROBLASTS; ACTIVATION; EXPRESSION; RATS;

D O I：

10.3892/mmr.2017.7662

中图分类号：

R73 [肿瘤学];

学科分类号：

100214 [肿瘤学];

摘要：

Ras homolog family member A (RhoA) has been reported to be involved in numerous biological processes; however, the effects of RhoA on acute lung injury (ALI) have yet to be reported. The present study aimed to explore how RhoA affects cell viability, reactive oxygen species (ROS) activity and cell apoptosis in a cell model of lipopolysaccharide (LPS)-induced ALI. An MTT assay, flow cytometry, reverse transcription-quantitative polymerase chain reaction and western blotting were used to determine the effects of RhoA on cell viability, apoptosis and ROS activity. The results demonstrated that RhoA inactivation was able to promote cell viability, and decrease apoptosis and ROS activity of LPS-treated cells. The results of western blotting indicated that RhoA activated the downstream Wnt/beta-catenin signaling pathway and inhibited the expression of apoptotic factors. These findings suggested that RhoA may be involved in ALI progression and could be a novel therapeutic target for this disease.

引用

收藏

页码：8501 / 8506

页数：6

相关论文

共 33 条

[1]

The Predictive Value of Von Willebrand Factor Antigen Plasma Levels in Children With Acute Lung Injury [J].

Abo El Ezz, Ahmed Abd El Basset ;

Abd El Hafez, Maher Ahmed ;

El Amrousy, Doaa Mohamed ;

Suliman, Ghada Abd El Momen .

PEDIATRIC PULMONOLOGY, 2017, 52 (01) :91-97

[2]

Acceleration of bone development and regeneration through the Wnt/β-catenin signaling pathway in mice heterozygously deficient for GSK-3β [J].

Arioka, Masaki ;

Takahashi-Yanaga, Fumi ;

Sasaki, Masanori ;

Yoshihara, Tatsuya ;

Morimoto, Sachio ;

Takashima, Akihiko ;

Mori, Yoshihide ;

Sasaguri, Toshiyuki .

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 2013, 440 (04) :677-682

[3]

A novel function for Cyclin A2: Control of cell invasion via RhoA signaling [J].

Arsic, Nikola ;

Bendris, Nawal ;

Peter, Marion ;

Begon-Pescia, Christina ;

Rebouissou, Cosette ;

Gadea, Gilles ;

Bouquier, Nathalie ;

Bibeau, Frederic ;

Lemmers, Benedicte ;

Blanchard, Jean Marie .

JOURNAL OF CELL BIOLOGY, 2012, 196 (01) :147-162

[4]

Bistability in the Rac1, PAK, and RhoA Signaling Network Drives Actin Cytoskeleton Dynamics and Cell Motility Switches [J].

Byrne, Kate M. ;

Monsefi, Naser ;

Dawson, John C. ;

Degasperi, Andrea ;

Bukowski-Wills, Jimi-Carlo ;

Volinsky, Natalia ;

Dobrzynski, Maciej ;

Birtwistle, Marc R. ;

Tsyganov, Mikhail A. ;

Kiyatkin, Anatoly ;

Kida, Katarzyna ;

Finch, Andrew J. ;

Carragher, Neil O. ;

Kolch, Walter ;

Nguyen, Lan K. ;

von Kriegsheim, Alex ;

Kholodenko, Boris N. .

CELL SYSTEMS, 2016, 2 (01) :38-48

[5]

Active and Passive Cigarette Smoking and Acute Lung Injury after Severe Blunt Trauma [J].

Calfee, Carolyn S. ;

Matthay, Michael A. ;

Eisner, Mark D. ;

Benowitz, Neal ;

Call, Mariah ;

Pittet, Jean-Francois ;

Cohen, Mitchell J. .

AMERICAN JOURNAL OF RESPIRATORY AND CRITICAL CARE MEDICINE, 2011, 183 (12) :1660-1665

[6]

DYSREGULATED RENIN-ANGIOTENSIN SYSTEM CONTRIBUTES TO ACUTE LUNG INJURY CAUSED BY HIND-LIMB ISCHEMIA-REPERFUSION IN MICE [J].

Chen, Li-Nan ;

Yang, Xiu-Hong ;

Nissen, Daniel H. ;

Chen, Yan-Yan ;

Wang, Li-Jun ;

Wang, Jian-Hui ;

Gao, Jun-Ling ;

Zhang, Lian-Yuan .

SHOCK, 2013, 40 (05) :420-429

[7]

High RhoA expression at the tumor front in clinically localized prostate cancer and association with poor tumor differentiation [J].

Chen, Weihua ;

Delongchamps, Nicolas Barry ;

Mao, Kaili ;

Beuvon, Frederic ;

Peyromaure, Michael ;

Liu, Zhongmin ;

Anh Tuan Dinh-Xuan .

ONCOLOGY LETTERS, 2016, 11 (02) :1375-1381

[8]

miR-146a is essential for lipopolysaccharide (LPS)-induced cross-tolerance against kidney ischemia/reperfusion injury in mice [J].

Dai, Yan ;

Jia, Ping ;

Fang, Yi ;

Liu, Hong ;

Jiao, Xiaoyan ;

He, John C. ;

Ding, Xiaoqiang .

SCIENTIFIC REPORTS, 2016, 6

[9]

Wnt-expressing rat embryonic fibroblasts suppress Apo2L/TRAIL-induced apoptosis of human leukemia cells [J].

Doubravska, Lenka ;

Simova, Sarka ;

Cermak, Lukas ;

Valenta, Tomas ;

Korinek, Vladimir ;

Andera, Ladislav .

APOPTOSIS, 2008, 13 (04) :573-587

[10]

GUANINE-NUCLEOTIDE EXCHANGE FACTOR H1 MEDIATES LIPOPOLYSACCHARIDE-INDUCED INTERLEUKIN 6 AND TUMOR NECROSIS FACTOR α EXPRESSION IN ENDOTHELIAL CELLS VIA ACTIVATION OF NUCLEAR FACTOR κB [J].

Guo, Feng ;

Xing, Ying ;

Zhou, Zengding ;

Dou, Yi ;

Tang, Jiajun ;

Gao, Chengjin ;

Huan, Jingning .

SHOCK, 2012, 37 (05) :531-538

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