Salivary Detection of H1N1 Virus: A Clinical Feasibility Investigation

被引：20

作者：

Bilder, L. ^{[1
]}

Machtei, E. E. ^{[1
]}

Shenhar, Y. ^{[2
]}

Kra-Oz, Z. ^{[2
]}

Basis, F. ^{[3
]}

机构：

[1] Technion Israel Inst Technol, Rappaport Fac Med, Sch Grad Dent, IL-31096 Haifa, Israel

[2] Technion Israel Inst Technol, Rappaport Fac Med, Virol Dept & Labs, IL-31096 Haifa, Israel

[3] Technion Israel Inst Technol, Rappaport Fac Med, Emergency Dept, IL-31096 Haifa, Israel

来源：

JOURNAL OF DENTAL RESEARCH | 2011年 / 90卷 / 09期

关键词：

nasopharyngeal swab; saliva; H1N1; emergency department; virus; diagnosis; TIME RT-PCR; RAPID DIAGNOSTIC-TEST; INFLUENZA-A; B VIRUSES; HCV RNA; INFECTION; ANTIGEN; FLUID; ASSAY; H5N1;

D O I：

10.1177/0022034511413283

中图分类号：

R78 [口腔科学];

学科分类号：

1003 ;

摘要：

The fast and efficient transportation among continents will continue to play a role in the spread of airborne pandemics. The objective of this study was to detect H1N1 virus in the saliva of individuals who visited the emergency department and were diagnosed as having H1N1 influenza. Nasopharyngeal swabs and saliva samples from those who presented to the emergency department with flu-like symptoms were sent to the laboratory. RNA was extracted from both samples. Real-time RT-PCR tests were performed, and the saliva and nasopharyngeal swab tests were compared. Samples were drawn from 26 individuals. A positive nasopharyngeal swab test and salivary test was found in 14 persons, and negative tests were found in 12 persons. Saliva sampling for H1N1 has excellent predictive value, is highly accurate and reliable, and is more convenient than the nasopharyngeal swab. Clinical trial with the Helsinki Committee at Rambam Health Care Campus, registration number 036309-RMB.

引用

页码：1136 / 1139

页数：4

共 30 条

[1] COMPARISON OF NASOPHARYNGEAL ASPIRATE AND NASOPHARYNGEAL SWAB SPECIMENS FOR RESPIRATORY SYNCYTIAL VIRUS DIAGNOSIS BY CELL-CULTURE, INDIRECT IMMUNOFLUORESCENCE ASSAY, AND ENZYME-LINKED-IMMUNOSORBENT-ASSAY [J].

AHLUWALIA, G ;

EMBREE, J ;

MCNICOL, P ;

LAW, B ;

HAMMOND, GW .

JOURNAL OF CLINICAL MICROBIOLOGY, 1987, 25 (05) :763-767

[2]

*AM COLL EM PHYS, 2010, NAT STRAT PLAN EM DE

[3] High Sensitivity of a Rapid Immunochromatographic Test for Detection of Influenza A Virus 2009 H1N1 in Nasopharyngeal Aspirates from Young Children [J].

Andresen, David N. ;

Kesson, Alison M. .

JOURNAL OF CLINICAL MICROBIOLOGY, 2010, 48 (07) :2658-2659

[4]

[Anonymous], GLOB AL RESP GAR PAN

[5]

BASIS F, 2005, ISR J EMERG MED, V5, P36

[6]

*CDC, 2009, INT GUID SPEC COLL P

[7] Comparative analytical sensitivities of six rapid influenza A antigen detection test kits for detection of influenza A subtypes H1N1H3N2 and H5N1 [J].

Chan, K. H. ;

Lam, S. Y. ;

Puthavathana, P. ;

Nguyen, T. D. ;

Long, H. T. ;

Pang, C. M. ;

Chan, K. M. ;

Cheung, C. Y. ;

Seto, W. H. ;

Peiris, J. S. M. .

JOURNAL OF CLINICAL VIROLOGY, 2007, 38 (02) :169-171

[8]

Chan LS, 2004, J TRAVEL MED, V11, P273, DOI 10.2310/7060.2004.19102

[9] DETECTION OF HEPATITIS-C VIRUS-RNA IN THE CELL FRACTION OF SALIVA BEFORE AND AFTER ORAL-SURGERY [J].

CHEN, M ;

YUN, ZB ;

SALLBERG, M ;

SCHVARCZ, R ;

BERGQUIST, I ;

BERGLUND, HB ;

SONNERBORG, A .

JOURNAL OF MEDICAL VIROLOGY, 1995, 45 (02) :223-226

[10] Duplex Real-Time Reverse Transcriptase PCR Assays for Rapid Detection and Identification of Pandemic (H1N1) 2009 and Seasonal Influenza A/H1, A/H3, and B Viruses [J].

Chidlow, Glenys ;

Harnett, Gerald ;

Williams, Simon ;

Levy, Avram ;

Speers, David ;

Smith, David W. .

JOURNAL OF CLINICAL MICROBIOLOGY, 2010, 48 (03) :862-866

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